CKAP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85671

Novus Biologicals, part of Bio-Techne
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NBP1-85671
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CKAP1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VSAPTVTVFISSSLNTFRSEKRYSRSLTIAEFKCKLELLVGSPASCMELELYGVDDKFYSKLDQEDALLGSYPVDDGCRIHVIDHSGARLGEYEDVSR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CKAP1 Antibody

Western Blot: CKAP1 Antibody [NBP1-85671]

Western Blot: CKAP1 Antibody [NBP1-85671]

Western Blot: CKAP1 Antibody [NBP1-85671] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: CKAP1 Antibody [NBP1-85671]

Immunocytochemistry/ Immunofluorescence: CKAP1 Antibody [NBP1-85671]

Immunocytochemistry/Immunofluorescence: CKAP1 Antibody [NBP1-85671] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
Immunohistochemistry-Paraffin: CKAP1 Antibody [NBP1-85671]

Immunohistochemistry-Paraffin: CKAP1 Antibody [NBP1-85671]

Immunohistochemistry-Paraffin: CKAP1 Antibody [NBP1-85671] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.
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Applications for CKAP1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CKAP1

CKAP1 binds to alpha-tubulin folding intermediates after their interaction with cytosolic chaperonin in the pathwayleading from newly synthesized tubulin to properly folded heterodimer. Involved in regulation of tubulin heterodimerdissociation. May function as

Alternate Names

CG22Tubulin-specific chaperone B, CKAP1MGC14625, CKAPI, cytoskeleton associated protein 1, Cytoskeleton-associated protein 1Cytoskeleton-associated protein CKAPI, tubulin folding cofactor B, tubulin-folding cofactor B

Gene Symbol

TBCB

Additional CKAP1 Products

Product Documents for CKAP1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CKAP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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