CKAP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00264

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CKAP1 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human CKAP1 (Uniprot#: Q99426; Met1-Ile244)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for CKAP1 Antibody

Western Blot: CKAP1 Antibody [NBP3-00264]

Western Blot: CKAP1 Antibody [NBP3-00264]

Western Blot: CKAP1 Antibody [NBP3-00264] - Anti-CKAP1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: A431 Whole Cell Lysate. Lane C: NCI-H1299 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 27 kDa. Observed band size: 35 kDa
Immunoprecipitation: CKAP1 Antibody [NBP3-00264]

Immunoprecipitation: CKAP1 Antibody [NBP3-00264]

Immunoprecipitation: CKAP1 Antibody [NBP3-00264] - CKAP1 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg HuT78 Whole Cell Lysate 2 ul anti-CKAP1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-CKAP1 rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 35 kDa. Observed band size: 35 kDa

Applications for CKAP1 Antibody

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

0.5-2 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CKAP1

CKAP1 binds to alpha-tubulin folding intermediates after their interaction with cytosolic chaperonin in the pathwayleading from newly synthesized tubulin to properly folded heterodimer. Involved in regulation of tubulin heterodimerdissociation. May function as

Alternate Names

CG22Tubulin-specific chaperone B, CKAP1MGC14625, CKAPI, cytoskeleton associated protein 1, Cytoskeleton-associated protein 1Cytoskeleton-associated protein CKAPI, tubulin folding cofactor B, tubulin-folding cofactor B

Gene Symbol

TBCB

Additional CKAP1 Products

Product Documents for CKAP1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CKAP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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