CKIP-1 Antibody - BSA Free

Western Blot: CKIP-1 AntibodyBSA Free [NBP1-76238]

Western Blot: CKIP-1 Antibody [NBP1-76238] - Human lung tissue lysate with CKIP1 antibody at (A) 1 and (B) 2 ug/mL.

Western Blot: CKIP-1 Antibody - BSA Free [NBP1-76238] -

Western Blot: CKIP-1 Antibody - BSA Free [NBP1-76238] - CKIP-1 directly interacts with Smurf1 to promote Smurf1 degradation.DF-1 chicken fibroblasts were transfected with the indicated constructs & then immunostained using anti-FLAG & anti-V5 antibodies. (A) Localization of FLAG-tagged CKIP-1 & V5-tagged Smurf1 alone. (B) Schematics for CKIP-1 FLAG constructs highlighting the membrane-binding PH domain (red) & Smurf1-binding LZ (blue) followed by a C-terminal FLAG tag (yellow). (C) Full-length CKIP-1 FLAG was cotransfected with full-length V5-Smurf1 or V5-Smurf1 C700A dominant-negative mutant. In addition, CKIP-1 FLAG truncation mutants were cotransfected with full-length V5-Smurf1, showing that the PH domain is required for efficient membrane localization, & the LZ domain is required for Smurf1 colocalization. Arrows indicate membrane localization. Scale bars represent 10 μm. (D) Chick embryos were electroporated at HH4 with V5-Smurf1 & CKIP-1 FLAG & then stained for V5 or FLAG at HH8 & imaged in cryosection. Scale bars represent 25 μm. (E) Western blot analysis of HH7 whole-embryo lysates electroporated with constructs indicated & probed for V5, pSmad1/5/8, & RFP. Bar graphs represent normalized V5 & pSmad1/5/8 staining intensity means ± SEM from 3 replicates. Underlying data can be found in S1 Data. P values from two-tailed Student t test. CKIP-1, casein kinase interacting protein 1; HH, Hamburger-Hamilton stage; LZ, leucine zipper motif; NC; neural crest domain; NS, not significant; PH, pleckstrin homology; pSmad1/5/8, phospho-Smads 1/5/8; RFP, red fluorescent protein; Smurf1, Smad ubiquitin regulatory factor 1. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29949573), licensed under a CC-BY license. Not internally tested by Novus Biologicals.