Clathrin light chain + heavy chain Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-05035
Key Product Details
Species Reactivity
Validated:
Human, Rat
Cited:
Rat
Predicted:
Bovine (100%), Canine (100%), Mouse (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence CDFNPKSSKQAKD corresponding to internal region according to NP_009027.1, NP_001070145.1, NP_001825.1, NP_009028.1.
Epitope
internal region
Reactivity Notes
Use in Rat reported in scientific literature (PMID:24876496).
Specificity
This antibody is expected to recognize all reported isoforms of light chains a and b.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Clathrin light chain + heavy chain Antibody
![Western Blot: Clathrin light chain + heavy chain Antibody [NBP1-05035]](https://resources.bio-techne.com/images/products/Clathrin-light-chain-+-heavy-chain-Antibody-Western-Blot-NBP1-05035-img0004.jpg "Western Blot: Clathrin light chain + heavy chain Antibody [NBP1-05035]")
Western Blot: Clathrin light chain + heavy chain Antibody [NBP1-05035]
Western Blot: Clathrin light chain + heavy chain Antibody [NBP1-05035] - (0.1ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.![Immunohistochemistry-Paraffin: Clathrin light chain + heavy chain Antibody [NBP1-05035]](https://resources.bio-techne.com/images/products/Clathrin-light-chain-+-heavy-chain-Antibody-Immunohistochemistry-Paraffin-NBP1-05035-img0003.jpg "Immunohistochemistry-Paraffin: Clathrin light chain + heavy chain Antibody [NBP1-05035]")
Immunohistochemistry-Paraffin: Clathrin light chain + heavy chain Antibody [NBP1-05035]
Immunohistochemistry-Paraffin: Clathrin light chain + heavy chain Antibody [NBP1-05035] - (2.5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Applications for Clathrin light chain + heavy chain Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
2.5 ug/ml
Peptide ELISA
Detection limit 1:32000
Western Blot
0.1-0.3 ug/ml
Application Notes
Western blot: Approx. 35kDa and 22-23kDa bands observed in Human Brain (Frontal Cortex) lysates (calculated MW of 23.2kDa according to NP_001825.1). The observed molecular weight of ~35kDa corresponds to earlier findings in literature with different antibodies (Okamoto et al, Am J Physiol Cell Physiol. 2000 Sep;279(3):C833-51.; PMID: 10942733).Primary incubation was 1 hour.
IHC: Paraffin embedded Human Spleen.
IHC: Paraffin embedded Human Spleen.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Clathrin light chain + heavy chain
Alternate Names
clathrin light chain A, clathrin, light chain A, LCA, light polypeptide (Lca)
Gene Symbol
CLTA
UniProt
Additional Clathrin light chain + heavy chain Products
Product Documents for Clathrin light chain + heavy chain Antibody
Product Specific Notices for Clathrin light chain + heavy chain Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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