Claudin-12 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87450
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Predicted:
Rat (91%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SLPSPFWQPLYSHPPSMHTYSQPYSARSRLSAIEIDIPVVSHTT
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Claudin-12 Antibody
Western Blot: Claudin-12 Antibody [NBP1-87450]
Western Blot: Claudin-12 Antibody [NBP1-87450] - Increased CLDN-5 and decreased CLDN-12 expression in the cortex of ASD subjects. a Brain tissues were lysed and immunoblotted with anti-claudin-12, SMA or actin antibody. b Densitometry analysis of the results from the western blots is shown, where the data are normalized against SMA expression and are expressed as the mean +/- standard error. Quantitative results represent the average of three independent experiments. ****p < 0.0001. Image collected and cropped by CiteAb from the following publication (https://molecularautism.biomedcentral.com/articles/10.1186/s13229-016-0110-z) licensed under a CC-BY license.Immunohistochemistry-Paraffin: Claudin-12 Antibody [NBP1-87450]
Immunohistochemistry-Paraffin: Claudin-12 Antibody [NBP1-87450] - Staining of human pancreas shows moderate cytoplasmic and membranous positivity in exocrine glandular cells along with moderate cytoplasmic staining in islets of LangerhansWestern Blot: Claudin-12 Antibody [NBP1-87450]
Western Blot: Claudin-12 Antibody [NBP1-87450] - Analysis in human liver tissue.Applications for Claudin-12 Antibody
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
0.04-0.4 ug/ml
Application Notes
WB reported in scientific literature (PMID: 29986305). For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Claudin-12
Alternate Names
Claudin12, CLDN12
Gene Symbol
CLDN12
Additional Claudin-12 Products
Product Documents for Claudin-12 Antibody
Product Specific Notices for Claudin-12 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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