Claudin-14 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1070
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Predicted:
Bovine (100%), Canine (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Validated:
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-SATHSGYRLNDYV corresponding to C-Terminus according to NP_036262.1.
Reactivity Notes
Predicted cross-reactivity based on sequence identity: Rat, Canine, Porcine, Bovine. Mouse reactivity reported in scientific literature (PMID: 21454667).
Localization
Cell Membrane
Specificity
Reported variants represent identical protein (NP_036262.1; NP_652763.1; NP_001139551.1; NP_001139550.1 and NP_001139549.1).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Claudin-14 Antibody
![Western Blot: Claudin-14 Antibody [NB100-1070]](https://resources.bio-techne.com/images/products/Claudin-14-Antibody-Western-Blot-NB100-1070-img0003.jpg "Western Blot: Claudin-14 Antibody [NB100-1070]")
Western Blot: Claudin-14 Antibody [NB100-1070]
Western Blot: Claudin-14 Antibody [NB100-1070] - Staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Applications for Claudin-14 Antibody
Application
Recommended Usage
ELISA
1:4000
Western Blot
0.3 - 1 ug/ml
Application Notes
WB: Approx. 23 kDa band observed in human liver lysate (calculated MW of 25.7 kDa band according to NP_652763.1). IHC successful usage on mouse heart.
IHC: This antibody has been successfully used in IHC on mouse heart. Use in IHC-P reported in scientific literature (PMID: 21454667).
IHC: This antibody has been successfully used in IHC on mouse heart. Use in IHC-P reported in scientific literature (PMID: 21454667).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Claudin-14
Alternate Names
Claudin14, CLDN14, DFNB29
Gene Symbol
CLDN14
UniProt
Additional Claudin-14 Products
Product Documents for Claudin-14 Antibody
Product Specific Notices for Claudin-14 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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