Claudin-18 Antibody (25E3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26657

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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NBP3-26657-100ul
NBP3-26657-50ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # 25E3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Claudin-18 Antibodies »

Product Summary for Claudin-18 Antibody (25E3)

Immunogen

Recombinant Human Claudin-18 protein [UniProt P56856]

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Scientific Data Images for Claudin-18 Antibody (25E3)

Claudin-18 Antibody (25E3)

ELISA: Claudin-18 Antibody (25E3) [NBP3-26657] -

ELISA: Claudin-18 Antibody (25E3) [NBP3-26657] - The Binding Activity of Human Claudin-18.2 with Claudin-18 Antibody.
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human Claudin-18.2 at 5 ug/mL can bind Claudin-18 Antibody, the EC50 is 2.947-8.782 ng/mL.
Claudin-18 Antibody (25E3)

Flow Cytometry: Claudin-18 Antibody (25E3) [NBP3-26657] -

Flow Cytometry: Claudin-18 Antibody (25E3) [NBP3-26657] - Untransfected KATO3 cells (green line), transfected Human CLDN18.2 KATO3 stable cells (red line) and transfected Human CLDN18.1 NIH-3T3 stable cells (blue line) were stained with anti-CLDN18.2 antibody (2ug/1*10^6 cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.

Applications for Claudin-18 Antibody (25E3)

Application
Recommended Usage

Flow Cytometry

1:50-1:200
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50% Glycerol, 0.01M PBS, PH 7.4

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Claudin-18

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is upregulated in patients with ulcerative colitis and highly overexpressed in infiltrating ductal adenocarcinomas. PKC/MAPK/AP-1 (protein kinase C/mitogen-activated protein kinase/activator protein-1) dependent pathway regulates the expression of this gene in gastric cells. Alternatively spliced transcript variants encoding different isoforms have been identified.

Alternate Names

Claudin18, CLDN18, SFTA5, SFTPJ

Gene Symbol

CLDN18

Additional Claudin-18 Products

Product Documents for Claudin-18 Antibody (25E3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Claudin-18 Antibody (25E3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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