Claudin-18.2 Antibody (zolbetuximab) - Chimeric

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-28796

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Functional

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # zolbetuximab

Concentration

LYOPH mg/ml

View all Claudin-18.2 Antibodies »

Product Specifications

Immunogen

CLDN18.2

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Endotoxin Level

< 0.001EU/ug,determined by LAL method.

Description

Expressed from CHO. The heavy chain type is huIgG1, and the light chain type is hukappa. It has a predicted MW of 145.5 kDa.

Also known as 'zolbetuximab'.

Upon receipt, store immediately at -20C or lower for 24 months in a lyophilized state. - 80C for 3 months after reconstitution. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Claudin-18.2 Antibody (zolbetuximab) - Chimeric

Claudin-18.2 Antibody (zolbetuximab) - Chimeric

ELISA: Claudin-18.2 Antibody (zolbetuximab) - Chimeric[NBP3-28796] -

Immobilized human CLDN18.2 VLP at 2 ug/mL can bind Claudin-18.2 Antibody (zolbetuximab) - Chimeric, EC50=0.07951 ug/mL.
Claudin-18.2 Antibody (zolbetuximab) - Chimeric

In vivo assay: Claudin-18.2 Antibody (zolbetuximab) - Chimeric[NBP3-28796] -

Zolbetuximab inhibited the tumor growth of CLDN18.2-MC38 (Mouse colorectal cancer cells) on C57BL/6N mice. The result showed significant anti-tumor effects, with an tumor inhibition rate (TGI) of 42.3% at 10 mpk at D25.

Applications for Claudin-18.2 Antibody (zolbetuximab) - Chimeric

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Reconstitute with sterile, distilled water to a final concentration of 1 mg/ml. Gently shake to solubilize completely. Do not vortex.

Formulation

Lyophilized from 25mM histidine, 8% sucrose, 0.01% Tween80 (pH6.2)

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Claudin-18.2

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is upregulated in patients with ulcerative colitis and highly overexpressed in infiltrating ductal adenocarcinomas. PKC/MAPK/AP-1 (protein kinase C/mitogen-activated protein kinase/activator protein-1) dependent pathway regulates the expression of this gene in gastric cells. Alternatively spliced transcript variants encoding different isoforms have been identified.

Alternate Names

claudin-18, SFTA5, SFTPJ, surfactant associated 5, surfactant associated protein J, surfactant, pulmonary associated protein J

Gene Symbol

CLDN18

UniProt

P56856 (Human)

Additional Claudin-18.2 Products

Product Documents for Claudin-18.2 Antibody (zolbetuximab) - Chimeric

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Claudin-18.2 Antibody (zolbetuximab) - Chimeric

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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