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Claudin-4 Antibody (10F4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26653

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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NBP3-26653-100ul
NBP3-26653-50ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG Clone # 10F4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Claudin-4 Antibody (10F4)

Immunogen

A synthesized peptide derived from Human Claudin-4 [UniProt O14493]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Scientific Data Images for Claudin-4 Antibody (10F4)

Claudin-4 Antibody (10F4)

Flow Cytometry: Claudin-4 Antibody (10F4) [NBP3-26653] -

Flow Cytometry: Claudin-4 Antibody (10F4) [NBP3-26653] - Overlay Peak curve showing MCF7 cells stained with Claudin-4 Antibody (10F4) (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45 min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG (H+L) at 1/200 dilution for 35 min at 4C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

Applications for Claudin-4 Antibody (10F4)

Application
Recommended Usage

Flow Cytometry

1:50-1:200
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50% Glycerol, 0.01M PBS, PH 7.4

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Claudin-4

The Claudin superfamily consists of many structurally related proteins in humans. These proteins are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Three classes of proteins are known to localize to tight junctions, including the Claudins, Occludin and junction adhesion molecule (JAM). Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. Claudin expression is highly restricted to specific regions of different tissues and variations of Claudin expression may have an important role in transcellular transport through tight junctions. In rat liver, claudin-3 is uniformly expressed, whereas in the pancreas, claudin-3 is expressed in junctions of the duct epithelia and junctions of acinar cells. Claudin-3 binds the peptide toxin Clostridium perfringens enterotoxin (CPE) at the cell surface via the second extracellular loop of claudin-3. The gene encoding human claudin-3 maps to chromosome 7q11.23.

Alternate Names

Claudin4, CLDN4, CPE-R, CPETR1, WBSCR8

Gene Symbol

CLDN4

Additional Claudin-4 Products

Product Documents for Claudin-4 Antibody (10F4)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Claudin-4 Antibody (10F4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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