Claudin-4 Antibody [Janelia Fluor® 525]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-41187JF525

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (94%), Mouse (94%). Backed by our 100% Guarantee.

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Janelia Fluor 525

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Claudin-4 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human Claudin-4. The immunogen is located within the last 50 amino acids of Claudin-4. Amino Acid Sequence: PPRTDKPYSAKYSAAR

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (88%).

Specificity

At least four isoforms of Claudin-4 are known to exist; Claudin-4 antibody will detect all four isoforms. Human Claudin-4 has one isoform (209aa, 22kD). Mouse Claudin-4 has one isoform (210aa, 22kD) and Rat Claudin-4 also has one isoform (210aa, 22kD). NBP2-41187 can detect human, mouse and rat.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for Claudin-4 Antibody [Janelia Fluor® 525]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Claudin-4

The Claudin superfamily consists of many structurally related proteins in humans. These proteins are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Three classes of proteins are known to localize to tight junctions, including the Claudins, Occludin and junction adhesion molecule (JAM). Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. Claudin expression is highly restricted to specific regions of different tissues and variations of Claudin expression may have an important role in transcellular transport through tight junctions. In rat liver, claudin-3 is uniformly expressed, whereas in the pancreas, claudin-3 is expressed in junctions of the duct epithelia and junctions of acinar cells. Claudin-3 binds the peptide toxin Clostridium perfringens enterotoxin (CPE) at the cell surface via the second extracellular loop of claudin-3. The gene encoding human claudin-3 maps to chromosome 7q11.23.

Alternate Names

Claudin4, CLDN4, CPE-R, CPETR1, WBSCR8

Gene Symbol

CLDN4

Additional Claudin-4 Products

Product Documents for Claudin-4 Antibody [Janelia Fluor® 525]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Claudin-4 Antibody [Janelia Fluor® 525]



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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