Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # S258-5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

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Product Summary for CLCN3 Antibody (S258-5)

Immunogen

Synthetic peptide amino acids 98-115 (cytoplasmic N-terminus) of rat Clcn3

Localization

Membrane , Endosome , Endosome membrane , Cytoplasmic Vesicle , Secretory Vesicle Membrane

Specificity

Detects 90kDa. Does not cross-react with Clcn4 or Clcn5 (based on KO validation results).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CLCN3 Antibody (S258-5)

Western Blot: CLCN3 Antibody (S258-5) [NBP2-59379]

Western Blot: CLCN3 Antibody (S258-5) [NBP2-59379]

Western Blot: CLCN3 Antibody (S258-5) [NBP2-59379] - Western Blot analysis of Rat Brain Membrane showing detection of ~90 kDa CIcn3 protein using Mouse Anti-CIcn3 Monoclonal Antibody, Clone S258-5 (NBP2-59379). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-CIcn3 Monoclonal Antibody (NBP2-59379) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~90 kDa.
Immunocytochemistry/ Immunofluorescence: CLCN3 Antibody (S258-5) [NBP2-59379]

Immunocytochemistry/ Immunofluorescence: CLCN3 Antibody (S258-5) [NBP2-59379]

Immunocytochemistry/Immunofluorescence: CLCN3 Antibody (S258-5) [NBP2-59379] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Clcn3 Monoclonal Antibody, Clone S258-5 (NBP2-59379). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Clcn3 Monoclonal Antibody (NBP2-59379) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Clcn3 Antibody (D) Composite.
Immunocytochemistry/ Immunofluorescence: CLCN3 Antibody (S258-5) [NBP2-59379]

Immunocytochemistry/ Immunofluorescence: CLCN3 Antibody (S258-5) [NBP2-59379]

Immunocytochemistry/Immunofluorescence: CLCN3 Antibody (S258-5) [NBP2-59379] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Clcn3 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane, Endosome, Endosome membrane, Cytoplasmic Vesicle, Secretory Vesicle Membrane. Magnification: 60X.
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Applications for CLCN3 Antibody (S258-5)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:1000

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: CLCN3

CLCN3 mediates the exchange of chloride ions against protons. Functions as antiporter and contributes to theacidification of the endosome and synaptic vesicle lumen, and may thereby affect vesicle trafficking and exocytosis.May play an important role in n

Alternate Names

chloride channel 3, Chloride channel protein 3, Chloride transporter ClC-3, CLC3, ClC-3, H(+)/Cl(-) exchange transporter 3

Gene Symbol

CLCN3

Additional CLCN3 Products

Product Documents for CLCN3 Antibody (S258-5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CLCN3 Antibody (S258-5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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