Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Predicted:

Orangutan (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

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Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 75 and 125 of human cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa using the numbering given in entry NP_001315.1 (GeneID 1477 ).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for cleavage stimulation factor Antibody

Western Blot: cleavage stimulation factor Antibody [NB100-60443]

Western Blot: cleavage stimulation factor Antibody [NB100-60443]

Western Blot: cleavage stimulation factor Antibody [NB100-60443] - Detection of human and mouse CSTF50 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CSTF50 antibody NB100-60443 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry-Paraffin: cleavage stimulation factor Antibody [NB100-60443]

Immunohistochemistry-Paraffin: cleavage stimulation factor Antibody [NB100-60443]

Immunohistochemistry-Paraffin: cleavage stimulation factor Antibody [NB100-60443] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CSTF50 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: cleavage stimulation factor Antibody [NB100-60443]

Immunohistochemistry-Paraffin: cleavage stimulation factor Antibody [NB100-60443]

Immunohistochemistry-Paraffin: cleavage stimulation factor Antibody [NB100-60443] - Detection of mouse CSTF50 by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-CSTF50 (NB100-60443). Detection: DAB
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Applications for cleavage stimulation factor Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:5000

Immunohistochemistry-Paraffin

1:1000 - 1:5000

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:1000 - 1:5000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Reviewed Applications

Read 1 review rated 5 using NB100-60443 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: cleavage stimulation factor

This gene encodes one of three subunits which combine to form cleavage stimulation factor (CSTF). CSTF is involved in the polyadenylation and 3'end cleavage of pre-mRNAs. Similar to mammalian G protein beta subunits, this protein contains transducin-like repeats. Several transcript variants with different 5' UTR, but encoding the same protein, have been found for this gene.

Alternate Names

CF-1 50 kDa subunit, Cleavage stimulation factor 50 kDa subunit, cleavage stimulation factor subunit 1, cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kD, cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa, CSTF 50 kDa subunit, CstF-50, CstFp50

Entrez Gene IDs

1477 (Human)

Gene Symbol

CSTF1

UniProt

NP_001315.1 (Human)

Additional cleavage stimulation factor Products

Product Documents for cleavage stimulation factor Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for cleavage stimulation factor Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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