CLEC4D/CLECSF8 Antibody (WEN42)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11193

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Product Datasheet / COA / SDS

Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Rat

Applications

Flow Cytometry, Functional, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2A Clone # WEN42

Concentration

0.1 mg/ml

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Product Summary for CLEC4D/CLECSF8 Antibody (WEN42)

Immunogen

BWZ.36 cell expressing chimeric CLEC4D/CLECSF8

Specificity

WEN42 does not bind to cells transfected with human MCL or with other APLEC receptors.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for CLEC4D/CLECSF8 Antibody (WEN42)

Flow Cytometry: CLEC4D/CLECSF8 Antibody (WEN42) [NBP3-11193]

Flow Cytometry: CLEC4D/CLECSF8 Antibody (WEN42) [NBP3-11193] - Detection of CLEC4D/CLECSF8 in transfected BW5147 cells. Red and blue line represent IgG1 Isotype control and CLEC4D/CLECSF8 stained cells respectively.

Applications for CLEC4D/CLECSF8 Antibody (WEN42)

Application

Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

For immunohistochemistry, Flow Cytometry, immunoprecipitation and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Endotoxin Level

<24 EU/mg

Formulation

0.2 um filtered solution in PBS, 0.1% BSA

Preservative

No Preservative

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CLEC4D/CLECSF8

The monoclonal antibody WEN42 recognizes rat macrophage C-type lectin (MCL). C-type lectin receptors (CLRs) have recently been identified as a fourth family of pattern-recognition receptors for pathogen associated molecular patterns, following TLRs, Nod-like receptors and RIG-I-like receptors. MCL is a membrane surface receptor encoded by the Antigen Presenting Lectin-like gene Complex (APLEC).The complex consists of seven related C-type lectin receptor genes, namely, Dendritic Cell Activating Receptor (DCAR), Dendritic Cell Inhibitory Receptor 1, 2, 3 and 24 (DCIR), Macrophage C-type lectin (MCL) and Macrophage inducible C-type lectin (Mincle). MCL is a type II transmembrane protein with a single extracellular C-terminal C-type lectin-like domain. C-type lectins are functionally diverse. Human MCL is expressed on macrophages (synovial, peritoneal, and blood monocyte-derived), Langerhans cells and on neutrophils, monocytes and immature and mature DCs. In rats, expression of MCL has been detected on macrophages, neutrophils, DCs, B cells, and T cells. Their presence on the surface of immune cells and their potential for recognizing polysaccharide structures suggests a central role as pattern-recognition receptors (PRR) in the innate immune system. Despite the growing amount of data describing expression and function of the APLEC receptors, very little has been reported about MCL in general and the rat MCL in particular. Studies investigating the role of MCL has demonstrated that MCL is capable of inducing phagocytosis, cytokine production and oxidative burst, suggesting an activating role for this protein in the immune response. WEN42 does not bind to cells transfected with human MCL or with other APLEC receptors.

Long Name

C-type Lectin Domain Family 4, Member D

Alternate Names

CD368, CLEC6, CLECSF8, Mpcl

Gene Symbol

CLEC4D

Additional CLEC4D/CLECSF8 Products

Product Documents for CLEC4D/CLECSF8 Antibody (WEN42)

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COA

SDS

Product Specific Notices for CLEC4D/CLECSF8 Antibody (WEN42)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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