CLEC4E Antibody (WEN43)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11192

Novus Biologicals, part of Bio-Techne
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NBP3-11192
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Key Product Details

Species Reactivity

Rat

Applications

Flow Cytometry, Functional

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # WEN43

Concentration

0.1 mg/ml

View all CLEC4E Antibodies »

Product Specifications

Immunogen

Rat CLEC4E-Fc gamma 2b fusion protein

Specificity

WEN43 does not bind to other receptors in the rat APLEC family.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<24 EU/mg

Applications for CLEC4E Antibody (WEN43)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
For Flow Cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um filtered solution in PBS, 0.1% BSA

Preservative

No Preservative

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CLEC4E

The monoclonal antibody WEN43 recognizes rat macrophage inducible C-type lectin (Mincle). This receptor is a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signaling, glycoprotein turnover, and roles in inflammation and immune response. For instance, Mincle is involved in mediating inflammatory responses to necrotic cells and is a pathogen sensor for Mycobacterium tuberculosis. It is a member of the Dendritic Cell Inhibitory Receptor (DCIR) group of C-type lectin receptors (CLRs), which are expressed mainly by antigen presenting cells and are encoded in a cluster in the natural killer gene complex. CLRs play crucial roles in defense against pathogens and recognize a variety of carbohydrate moieties. Ligand binding to CLRs leads to the activation of NF-kB via a Syk/CARD9 signaling module. Mincle was originally identified as a lipopolysaccharide-inducible protein in macrophages and has been shown to stimulate inflammatory responses to fungi, although the ligands and signaling pathways involved have not been defined. WEN43 does not bind to other receptors in the rat APLEC family.

Long Name

C-type Lectin Domain Family 4, Member E

Alternate Names

CLECSF9, MINCLE

Gene Symbol

CLEC4E

Additional CLEC4E Products

Product Documents for CLEC4E Antibody (WEN43)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CLEC4E Antibody (WEN43)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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