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CLIC4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85574

Novus Biologicals, part of Bio-Techne
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NBP1-85574
NBP1-85574-25ul

Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: THPPFITFNSEVKTDVNKIEEFLEEVLCPPKYLKLSPKHPESNTAGMDIFAKFSAYIKNSRPEANEALERGLLKTLQKLDEYLNSPLPDEIDENSMEDIKFSTRKFLDGNEMTLADCNLLPKLHIVKVVAKKYRNFDIPKEMTGI

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CLIC4 Antibody

Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP1-85574]

Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP1-85574]

Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP1-85574] - Staining in human kidney and skeletal muscle tissues using anti-CLIC4 antibody. Corresponding CLIC4 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP1-85574]

Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP1-85574]

Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP1-85574] - Staining of human colon, endometrium, kidney and skeletal muscle using Anti-CLIC4 antibody NBP1-85574 (A) shows similar protein distribution across tissues to independent antibody NBP2-49321 (B).
Western Blot: CLIC4 Antibody [NBP1-85574]

Western Blot: CLIC4 Antibody [NBP1-85574]

Western Blot: CLIC4 Antibody [NBP1-85574] - Analysis in human cell lines SK-MEL-30 and MCF-7 using anti-CLIC4 antibody. Corresponding CLIC4 RNA-seq data are presented for the same cell lines. Loading control: anti-PFN1.

Applications for CLIC4 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CLIC4

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells). [provided by RefSeq]

Alternate Names

chloride intracellular channel 4, chloride intracellular channel 4 like, chloride intracellular channel protein 4, CLIC4L, DKFZp566G223, FLJ38640, H1, huH1, Intracellular chloride ion channel protein p64H1, MTCLIC, p64H1

Gene Symbol

CLIC4

Additional CLIC4 Products

Product Documents for CLIC4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CLIC4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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