CLOCK Antibody (HL1099)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13718
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Knockout/Knockdown, Biological Validation
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1099
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human CLOCK. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Centrifuge briefly prior to opening.
Scientific Data Images for CLOCK Antibody (HL1099)
![Western Blot: CLOCK Antibody (HL1099) [NBP3-13718]](https://resources.bio-techne.com/images/products/CLOCK-Antibody-HL1099-Western-Blot-NBP3-13718-img0001.jpg "Western Blot: CLOCK Antibody (HL1099) [NBP3-13718]")
Western Blot: CLOCK Antibody (HL1099) [NBP3-13718]
Western Blot: CLOCK Antibody (HL1099) [NBP3-13718] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CLOCK antibody [HL1099] (NBP3-13718) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: CLOCK Antibody (HL1099) [NBP3-13718] -
Immunocytochemistry/ Immunofluorescence: CLOCK Antibody (HL1099) [NBP3-13718] - CLOCK antibody [HL1099] detects CLOCK protein by immunofluorescent analysis.Sample: DIV9 rat hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CLOCK stained by CLOCK antibody [HL1099] (NBP3-13718) diluted at 1:500.Red: Tau, a Axon marker, stained by Tau antibody [GT287] diluted at 1:500.Blue: Fluoroshield with DAPI .
Immunohistochemistry-Paraffin: CLOCK Antibody (HL1099) [NBP3-13718] -
Immunohistochemistry-Paraffin: CLOCK Antibody (HL1099) [NBP3-13718] - CLOCK antibody [HL1099] detects CLOCK protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.CLOCK stained by CLOCK antibody [HL1099] (NBP3-13718) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 minApplications for CLOCK Antibody (HL1099)
Application
Recommended Usage
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CLOCK
CLOCK levels peak during the late night and the early part of the subjective day. Mutations in CLOCK causes defective transcriptional activity and lengthens the circadian period, leading to abnormal circadian behavior.
CLOCK antibodies are useful tools for research on circadian rythmns, sleep biology and hormonal cycles.
Long Name
Circadian Locomoter Output Cycles Kaput
Alternate Names
BHLHE8, circadian locomoter output cycles protein kaput, Class E basic helix-loop-helix protein 8, clock (mouse) homolog, clock homolog (mouse), EC 2.3.1.48, hCLOCK, KAT13D, KIAA0334bHLHe8circadian locomoter output cycles kaput protein
Gene Symbol
CLOCK
Additional CLOCK Products
Product Documents for CLOCK Antibody (HL1099)
Product Specific Notices for CLOCK Antibody (HL1099)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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