CLPP Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89557
![Western Blot: CLPP Antibody [NBP1-89557]](https://resources.bio-techne.com/images/products/CLPP-Antibody-Western-Blot-NBP1-89557-img0007.jpg "Western Blot: CLPP Antibody [NBP1-89557]")
![Western Blot: CLPP Antibody [NBP1-89557]](https://resources.bio-techne.com/images/products/CLPP-Antibody-Western-Blot-NBP1-89557-img0011.jpg "Western Blot: CLPP Antibody [NBP1-89557]")
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot
Cited:
Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: MGSLLLAAGTPGMRHSLPNSRIMIHQPSGGARGQATDIAIQAEEIMKLKKQLYNIYAKHTKQSLQVIESAMERDRYMSPMEAQEFGILDKVLVHPPQDGEDEPTLVQKEPVEAAPAAEPVPA
Reactivity Notes
Reactivity reported in scientific literature (PMID: 22361696)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CLPP Antibody
![Immunocytochemistry/ Immunofluorescence: CLPP Antibody [NBP1-89557]](https://resources.bio-techne.com/images/products/CLPP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89557-img0010.jpg "Immunocytochemistry/ Immunofluorescence: CLPP Antibody [NBP1-89557]")
Immunocytochemistry/ Immunofluorescence: CLPP Antibody [NBP1-89557]
Immunocytochemistry/Immunofluorescence: CLPP Antibody [NBP1-89557] - Staining of human cell line U-251 MG shows localization to mitochondria. Antibody staining is shown in green.![Immunohistochemistry-Paraffin: CLPP Antibody [NBP1-89557]](https://resources.bio-techne.com/images/products/CLPP-Antibody-Immunohistochemistry-Paraffin-NBP1-89557-img0015.jpg "Immunohistochemistry-Paraffin: CLPP Antibody [NBP1-89557]")
Immunohistochemistry-Paraffin: CLPP Antibody [NBP1-89557]
Immunohistochemistry-Paraffin: CLPP Antibody [NBP1-89557] - Staining of human small intestine shows strong granular cytoplasmic positivity in glandular cells.![Immunohistochemistry-Paraffin: CLPP Antibody [NBP1-89557]](https://resources.bio-techne.com/images/products/CLPP-Antibody-Immunohistochemistry-Paraffin-NBP1-89557-img0012.jpg "Immunohistochemistry-Paraffin: CLPP Antibody [NBP1-89557]")
![Immunohistochemistry-Paraffin: CLPP Antibody [NBP1-89557]](https://resources.bio-techne.com/images/products/CLPP-Antibody-Immunohistochemistry-Paraffin-NBP1-89557-img0013.jpg "Immunohistochemistry-Paraffin: CLPP Antibody [NBP1-89557]")
![Immunohistochemistry-Paraffin: CLPP Antibody [NBP1-89557]](https://resources.bio-techne.com/images/products/CLPP-Antibody-Immunohistochemistry-Paraffin-NBP1-89557-img0014.jpg "Immunohistochemistry-Paraffin: CLPP Antibody [NBP1-89557]")
Applications for CLPP Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200-1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. Use in IHC-Frozen was reported in scientific literature (PMID: 30877431).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CLPP
Alternate Names
ATP-dependent protease ClpAP, proteolytic subunit, human, ClpP (caseinolytic protease, ATP-dependent, proteolytic subunit, E. coli)homolog, ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog (E.coli), ClpP caseinolytic protease, ATP-dependent, proteolytic subunit homolog, ClpP caseinolytic protease, ATP-dependent, proteolytic subunit homolog (E.coli), EC 3.4.21.92, Endopeptidase Clp, putative ATP-dependent Clp protease proteolytic subunit, mitochondrial
Gene Symbol
CLPP
Additional CLPP Products
Product Documents for CLPP Antibody
Product Specific Notices for CLPP Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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