CMP kinase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00262

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CMP kinase Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human CMP kinase (Accession#: P30085; Met1-Gly196)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for CMP kinase Antibody

Western Blot: CMP kinase Antibody [NBP3-00262]

Western Blot: CMP kinase Antibody [NBP3-00262]

Western Blot: CMP kinase Antibody [NBP3-00262] - Anti-CMP kinase rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 22 kDa. Observed band size: 23 kDa
Immunoprecipitation: CMP kinase Antibody [NBP3-00262]

Immunoprecipitation: CMP kinase Antibody [NBP3-00262]

Immunoprecipitation: CMP kinase Antibody [NBP3-00262] - CMP kinase was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate 2 ul anti-CMP kinase rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-CMP kinase rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 22 kDa. Observed band size: 22 kDa

Applications for CMP kinase Antibody

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CMP kinase

Uridine monophosphate (UMP)/cytidine monophosphate (CMP) kinase (EC 2.7.4.4) catalyzes the phosphoryl transfer from ATP to UMP, CMP, and deoxy-CMP (dCMP), resulting in the formation of ADP and the corresponding nucleoside diphosphate. These nucleoside diphosphates are required for cellular nucleic acid synthesis (Liou et al., 2002 [PubMed 11912132]).[supplied by OMIM]

Alternate Names

CMKEC 2.7.4.14, CMPKUMKUMP-CMP kinase, cytidine monophosphate (UMP-CMP) kinase 1, cytosolic, Cytidine monophosphate kinase, Cytidylate kinaseUMP/CMP kinase, Deoxycytidylate kinase, EC 2.7.4, UMP/CMPK, UMP-CMPK, UMPKUCK, Uridine monophosphate kinase, Uridine monophosphate/cytidine monophosphate kinase

Gene Symbol

CMPK1

Additional CMP kinase Products

Product Documents for CMP kinase Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CMP kinase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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