CNOT10 Antibody (PCRP-CNOT10-1D5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13921
![Immunocytochemistry/ Immunofluorescence: CNOT10 Antibody (PCRP-CNOT10-1D5) [NBP3-13921]](https://resources.bio-techne.com/images/products/CNOT10-Antibody-PCRP-CNOT10-1D5-Immunocytochemistry-Immunofluorescence-NBP3-13921-img0002.jpg "Immunocytochemistry/ Immunofluorescence: CNOT10 Antibody (PCRP-CNOT10-1D5) [NBP3-13921]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2C Clone # PCRP-CNOT10-1D5
Concentration
0.2 mg/ml
Product Summary for CNOT10 Antibody (PCRP-CNOT10-1D5)
Immunogen
Recombinant full-length human CNOT10 protein (Uniprot: Q9H9A5)
Localization
Cytoplasm, Mitochondrion and Nucleus, PML body
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2C
Theoretical MW
82.31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14266)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for CNOT10 Antibody (PCRP-CNOT10-1D5)
Immunocytochemistry/ Immunofluorescence: CNOT10 Antibody (PCRP-CNOT10-1D5) [NBP3-13921]
Immunocytochemistry/Immunofluorescence: CNOT10 Antibody (PCRP-CNOT10-1D5) [NBP3-13921] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using CNOT10 Antibody (PCRP-CNOT10-1D5) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).![Flow Cytometry: CNOT10 Antibody (PCRP-CNOT10-1D5) [NBP3-13921]](https://resources.bio-techne.com/images/products/CNOT10-Antibody-PCRP-CNOT10-1D5-Flow-Cytometry-NBP3-13921-img0001.jpg "Flow Cytometry: CNOT10 Antibody (PCRP-CNOT10-1D5) [NBP3-13921]")
Flow Cytometry: CNOT10 Antibody (PCRP-CNOT10-1D5) [NBP3-13921]
Flow Cytometry: CNOT10 Antibody (PCRP-CNOT10-1D5) [NBP3-13921] - Flow cytometric analysis of PFA-fixed HeLa cells. CNOT10 antibody (PCRP-CNOT10-1D5) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).![Protein Array: CNOT10 Antibody (PCRP-CNOT10-1D5) [NBP3-13921]](https://resources.bio-techne.com/images/products/CNOT10-Antibody-PCRP-CNOT10-1D5-Protein-Array-NBP3-13921-img0004.jpg "Protein Array: CNOT10 Antibody (PCRP-CNOT10-1D5) [NBP3-13921]")
Protein Array: CNOT10 Antibody (PCRP-CNOT10-1D5) [NBP3-13921]
Protein Array: CNOT10 Antibody (PCRP-CNOT10-1D5) [NBP3-13921] - Analysis of Protein Array containing more than 19,000 full-length human proteins using CNOT10 Antibody (PCRP-CNOT10-1D5).Applications for CNOT10 Antibody (PCRP-CNOT10-1D5)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunoprecipitation
1-2 ug/100-500 ug protein
Western Blot
1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA
Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: CNOT10
Alternate Names
CCR4-NOT transcription complex subunit 10, CCR4-NOT transcription complex, subunit 10, DKFZp434K115, FLJ12890, FLJ13165
Gene Symbol
CNOT10
UniProt
Additional CNOT10 Products
Product Documents for CNOT10 Antibody (PCRP-CNOT10-1D5)
Product Specific Notices for CNOT10 Antibody (PCRP-CNOT10-1D5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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