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CNOT3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-21396

Novus Biologicals, part of Bio-Techne
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NBP1-21396

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 525 and 575 of human CCR4-NOT transcription complex, subunit 3 using the numbering given in entry NP_055331.1 (GeneID 4849).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CNOT3 Antibody

Western Blot: CNOT3 Antibody [NBP1-21396]

Western Blot: CNOT3 Antibody [NBP1-21396]

Western Blot: CNOT3 Antibody [NBP1-21396] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NBP1-21396 used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate
Immunohistochemistry-Paraffin: CNOT3 Antibody [NBP1-21396]

Immunohistochemistry-Paraffin: CNOT3 Antibody [NBP1-21396]

Immunohistochemistry-Paraffin: CNOT3 Antibody [NBP1-21396] - Human testicular seminoma. Antibody used at a dilution of 1:200 (1ug/ml).
Western Blot: CNOT3 Antibody [NBP1-21396]

Western Blot: CNOT3 Antibody [NBP1-21396]

Western Blot: CNOT3 Antibody [NBP1-21396] - Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, and mouse TCMK-1 cells.

Applications for CNOT3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CNOT3

CNOT3 (CCR4-NOT transcription complex subunit 3) is a component of the CCR4-NOT core complex that functions as a general transcriptional regulator. In a genome-wide siRNA screen for genes essential for mouse embryonic stem cell self-renewal, CNOT3 was identified and found to co-occupy gene promoters with c-Myc and Zfx.

Alternate Names

CCR4-NOT transcription complex, subunit 3, KIAA0691CCR4-associated factor 3, LENG2yeast) homolog, Leukocyte receptor cluster member 2

Entrez Gene IDs

4849 (Human)

Gene Symbol

CNOT3

UniProt

Additional CNOT3 Products

Product Documents for CNOT3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CNOT3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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