CNOT4 Antibody (OTI1H2) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71212
![Immunohistochemistry: CNOT4 Antibody (OTI1H2) - Azide and BSA Free [NBP2-71212]](https://resources.bio-techne.com/images/products/CNOT4-Antibody-OTI1H2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71212-img0003.jpg "Immunohistochemistry: CNOT4 Antibody (OTI1H2) - Azide and BSA Free [NBP2-71212]")
![Western Blot: CNOT4 Antibody (OTI1H2)Azide and BSA Free [NBP2-71212]](https://resources.bio-techne.com/images/products/CNOT4-Antibody-OTI1H2-Azide-and-BSA-Free-Western-Blot-NBP2-71212-img0001.jpg "Western Blot: CNOT4 Antibody (OTI1H2)Azide and BSA Free [NBP2-71212]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI1H2
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Human recombinant protein fragment corresponding to amino acids 190-455 of human CNOT4 (NP_037448) produced in E.coli.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
70.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CNOT4 Antibody (OTI1H2) - Azide and BSA Free
Western Blot: CNOT4 Antibody (OTI1H2)Azide and BSA Free [NBP2-71212]
Western Blot: CNOT4 Antibody (OTI1H2) - Azide and BSA Free [NBP2-71212] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CNOT4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CNOT4.![Immunohistochemistry: CNOT4 Antibody (OTI1H2) - Azide and BSA Free [NBP2-71212]](https://resources.bio-techne.com/images/products/CNOT4-Antibody-OTI1H2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71212-img0006.jpg "Immunohistochemistry: CNOT4 Antibody (OTI1H2) - Azide and BSA Free [NBP2-71212]")
Immunohistochemistry: CNOT4 Antibody (OTI1H2) - Azide and BSA Free [NBP2-71212]
Immunohistochemistry: CNOT4 Antibody (OTI1H2) - Azide and BSA Free [NBP2-71212] - Staining of paraffin-embedded Human prostate tissue using anti-CNOT4 mouse monoclonal antibody.![Immunohistochemistry: CNOT4 Antibody (OTI1H2) - Azide and BSA Free [NBP2-71212]](https://resources.bio-techne.com/images/products/CNOT4-Antibody-OTI1H2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71212-img0002.jpg "Immunohistochemistry: CNOT4 Antibody (OTI1H2) - Azide and BSA Free [NBP2-71212]")
Immunohistochemistry: CNOT4 Antibody (OTI1H2) - Azide and BSA Free [NBP2-71212]
Immunohistochemistry: CNOT4 Antibody (OTI1H2) - Azide and BSA Free [NBP2-71212] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CNOT4 mouse monoclonal antibody.![Immunohistochemistry: CNOT4 Antibody (OTI1H2) - Azide and BSA Free [NBP2-71212]](https://resources.bio-techne.com/images/products/CNOT4-Antibody-OTI1H2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71212-img0004.jpg "Immunohistochemistry: CNOT4 Antibody (OTI1H2) - Azide and BSA Free [NBP2-71212]")
![Immunohistochemistry: CNOT4 Antibody (OTI1H2) - Azide and BSA Free [NBP2-71212]](https://resources.bio-techne.com/images/products/CNOT4-Antibody-OTI1H2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71212-img0005.jpg "Immunohistochemistry: CNOT4 Antibody (OTI1H2) - Azide and BSA Free [NBP2-71212]")
![Immunohistochemistry: CNOT4 Antibody (OTI1H2) - Azide and BSA Free [NBP2-71212]](https://resources.bio-techne.com/images/products/CNOT4-Antibody-OTI1H2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71212-img0007.jpg "Immunohistochemistry: CNOT4 Antibody (OTI1H2) - Azide and BSA Free [NBP2-71212]")
Applications for CNOT4 Antibody (OTI1H2) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
1:150
Western Blot
1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CNOT4
Alternate Names
CCR4-associated factor 4, CCR4-NOT transcription complex, subunit 4, E3 ubiquitin-protein ligase CNOT4, EC 6.3.2.-, NOT4yeast) homolog
Gene Symbol
CNOT4
Additional CNOT4 Products
Product Documents for CNOT4 Antibody (OTI1H2) - Azide and BSA Free
Product Specific Notices for CNOT4 Antibody (OTI1H2) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...