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CNOT7 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92571

Novus Biologicals, part of Bio-Techne
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NBP2-92571-0.1ml
NBP2-92571-0.02ml

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 90-160 of human CNOT7 (NP_037486.2). PGTSTWQFNFKFNLTEDMYAQDSIELLTTSGIQFKKHEEEGIETQYFAELLMTSGVVLCEGVKWLSFHSGY

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CNOT7 Antibody - Azide and BSA Free

Western Blot: CNOT7 AntibodyAzide and BSA Free [NBP2-92571]

Western Blot: CNOT7 AntibodyAzide and BSA Free [NBP2-92571]

Western Blot: CNOT7 Antibody [NBP2-92571] - Analysis of extracts of various cell lines, using CNOT7 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.
Immunohistochemistry-Paraffin: CNOT7 Antibody - Azide and BSA Free [NBP2-92571]

Immunohistochemistry-Paraffin: CNOT7 Antibody - Azide and BSA Free [NBP2-92571]

Immunohistochemistry-Paraffin: CNOT7 Antibody [NBP2-92571] - Immunohistochemistry of paraffin-embedded Human colon using CNOT7 Rabbit pAb (NBP2-92571) at dilution of 1:50 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: CNOT7 Antibody - Azide and BSA Free [NBP2-92571]

Immunohistochemistry-Paraffin: CNOT7 Antibody - Azide and BSA Free [NBP2-92571]

Immunohistochemistry-Paraffin: CNOT7 Antibody [NBP2-92571] - Immunohistochemistry of paraffin-embedded Mouse brain using CNOT7 Rabbit pAb (NBP2-92571) at dilution of 1:50 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Applications for CNOT7 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:200-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CNOT7

CNOT7 is encoded by this gene binds to an anti-proliferative protein, B-cell translocation protein 1, which negatively regulates cell proliferation. Binding of the two proteins, which is driven by phosphorylation of the anti-proliferative protein, causes signaling events in cell division that lead to changes in cell proliferation associated with cell-cell contact. The protein has both mouse and yeast orthologs. Alternate splicing of this gene results in two transcript variants encoding different isoforms.

Alternate Names

BTG1 binding factor 1, BTG1-binding factor 1, CAF-1, CAF1carbon catabolite repressor protein (CCR4)-associative factor 1, CCR4-associated factor 1, CCR4-NOT transcription complex subunit 7, CCR4-NOT transcription complex, subunit 7, hCAF-1

Gene Symbol

CNOT7

Additional CNOT7 Products

Product Documents for CNOT7 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CNOT7 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov