CNOT7 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12705

Novus Biologicals, part of Bio-Techne
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NBP3-12705-100ul
NBP3-12705-50ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CNOT7 Antibodies »

Product Summary for CNOT7 Antibody

Immunogen

Produced in rabbits immunized with E. coli-derived Human CNOT7 fragment

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Protein A & Antigen Affinity purified

Scientific Data Images for CNOT7 Antibody

Western Blot: CNOT7 Antibody [NBP3-12705]

Western Blot: CNOT7 Antibody [NBP3-12705]

Western Blot: CNOT7 Antibody [NBP3-12705] - Lane A: HepG2 Whole Cell LysateLane B: RT-4 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:33 kDaObserved band size:33 kDa
Immunoprecipitation: CNOT7 Antibody [NBP3-12705]

Immunoprecipitation: CNOT7 Antibody [NBP3-12705]

Immunoprecipitation: CNOT7 Antibody [NBP3-12705] - Lane A:0.5 mg RT-4 Whole Cell Lysate4 uL anti-CNOT7 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-CNOT7 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 33 kDaObserved band size :33 kDa

Applications for CNOT7 Antibody

Application
Recommended Usage

Immunoprecipitation

1-5ul/mg of lysate

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CNOT7

CNOT7 is encoded by this gene binds to an anti-proliferative protein, B-cell translocation protein 1, which negatively regulates cell proliferation. Binding of the two proteins, which is driven by phosphorylation of the anti-proliferative protein, causes signaling events in cell division that lead to changes in cell proliferation associated with cell-cell contact. The protein has both mouse and yeast orthologs. Alternate splicing of this gene results in two transcript variants encoding different isoforms.

Alternate Names

BTG1 binding factor 1, BTG1-binding factor 1, CAF-1, CAF1carbon catabolite repressor protein (CCR4)-associative factor 1, CCR4-associated factor 1, CCR4-NOT transcription complex subunit 7, CCR4-NOT transcription complex, subunit 7, hCAF-1

Gene Symbol

CNOT7

Additional CNOT7 Products

Product Documents for CNOT7 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CNOT7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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