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CNOT8 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15930

Novus Biologicals, part of Bio-Techne
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NBP2-15930

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Bovine (100%), Chicken (97%), Chimpanzee (100%), Mouse (99%), Rat (100%), Rhesus Macaque (100%), Xenopus (95%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human CNOT8. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (95%), Zebrafish (87%).

Localization

Cytoplasm; Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CNOT8 Antibody

CNOT8 Antibody

Western Blot: CNOT8 Antibody [NBP2-15930] -

Western Blot: CNOT8 Antibody [NBP2-15930] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CNOT8 antibody (NBP2-15930) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
CNOT8 Antibody

Immunocytochemistry/ Immunofluorescence: CNOT8 Antibody [NBP2-15930] -

Immunocytochemistry/ Immunofluorescence: CNOT8 Antibody [NBP2-15930] - CNOT8 antibody detects CNOT8 protein at nucleus and cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CNOT8 protein stained by CNOT8 antibody (NBP2-15930) diluted at 1:200.
Blue: Hoechst 33342 staining.

Applications for CNOT8 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CNOT8

Ubiquitous transcription factor required for a diverse set of processes. The CCR4-NOT complex functions as general transcription regulation complex

Alternate Names

CAF1, CAF2, CALIFCAF1-like protein, CALIFp, CCR4-NOT transcription complex subunit 8, CCR4-NOT transcription complex, subunit 8, hCAF1, PGK promoter directed over production, POP2CCR4-associated factor 8

Gene Symbol

CNOT8

UniProt

Additional CNOT8 Products

Product Documents for CNOT8 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CNOT8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.