CNPase Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-50058
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for CNPase Antibody
Immunogen
Full length human CNPase recombinant protein. [UniProt# P09543]
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CNPase Antibody
![Western Blot: CNPase Antibody [NBP2-50058]](https://resources.bio-techne.com/images/products/CNPase-Antibody-Western-Blot-NBP2-50058-img0004.jpg "Western Blot: CNPase Antibody [NBP2-50058]")
Western Blot: CNPase Antibody [NBP2-50058]
Western Blot: CNPase Antibody [NBP2-50058] - Analysis of different tissue lysates using rabbit pAb to CNP, NBP2-50058, dilution 1:5,000 in red: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, and [6] cow spinal cord. Double bands at 46, 48kDa mark correspond to isotypes of the CNP protein. The blot was simultaneously probed with mouse mAb to calretinin, dilution 1:2,000, in green. The single band at 29kDa corresponds to the calretinin protein.![Immunocytochemistry/ Immunofluorescence: CNPase Antibody [NBP2-50058]](https://resources.bio-techne.com/images/products/CNPase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-50058-img0003.jpg "Immunocytochemistry/ Immunofluorescence: CNPase Antibody [NBP2-50058]")
Immunocytochemistry/ Immunofluorescence: CNPase Antibody [NBP2-50058]
Immunocytochemistry/Immunofluorescence: CNPase Antibody [NBP2-50058] - Mixed neuron-glial cell cultures stained with NBP2-50058 (green) and monoclonal antibody against GFAP (red, NBP1-05197). The CNPase antibody stains strongly in oligodendrocytes, whereas GFAP labels the intermediate filament in astrocytes. Blue is DNA staining showing the nuclei of these and numerous other cells, most of which are neurons and which do not express either CNPase or GFAP.Applications for CNPase Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:1000 - 1:2000
Immunohistochemistry
1:1000 - 1:2000
Western Blot
1:1000 - 1:5000
Application Notes
In western blot, double bands may be detected at 46 and 48kDa, corresponding to the two isotypes of the CNP protein.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Supplied as serum
Preservative
0.035% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CNPase
Alternate Names
2', 3' cyclic nucleotide 3' phosphohydrolase, 2'-3'-cyclic nucleotide 3' phosphodiesterase, 2'-3'-cyclic-nucleotide 3'-phosphodiesterase, CNP1, CNPase, EC 3.1.4.37
Gene Symbol
CNP
UniProt
Additional CNPase Products
Product Documents for CNPase Antibody
Product Specific Notices for CNPase Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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