COBRA1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-05977

Novus Biologicals, part of Bio-Techne
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NBP1-05977
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all COBRA1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 530 and 580 of human cofactor of BRCA1 using the numbering given in entry NP_056271.2 (GeneID 25920).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for COBRA1 Antibody

Western Blot: COBRA1 Antibody [NBP1-05977]

Western Blot: COBRA1 Antibody [NBP1-05977]

Western Blot: COBRA1 Antibody [NBP1-05977] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NBP1-05977 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. COBRA1 was also immunoprecipitated by NBP1-05976, which recognizes an upstream epitope.
Immunohistochemistry-Paraffin: COBRA1 Antibody [NBP1-05977]

Immunohistochemistry-Paraffin: COBRA1 Antibody [NBP1-05977]

Immunohistochemistry-Paraffin: COBRA1 Antibody [NBP1-05977] - Human placenta. Antibody used at a dilution of 1:200 (1ug/ml).
Immunohistochemistry-Paraffin: COBRA1 Antibody [NBP1-05977]

Immunohistochemistry-Paraffin: COBRA1 Antibody [NBP1-05977]

Immunohistochemistry-Paraffin: COBRA1 Antibody [NBP1-05977] - human breast carcinoma. NBP1-05977 a dilution of 1:250.

Applications for COBRA1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:100

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: COBRA1

NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2; MIM 606026), either NELFC or NELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 [PubMed 12612062]).[supplied by OMIM]

Alternate Names

cofactor of BRCA1DKFZp586B0519, KIAA1182negative elongation factor B, NELFB, NELF-Bnegative elongation factor protein B

Entrez Gene IDs

25920 (Human)

Gene Symbol

COBRA1

UniProt

Q8WX92 (Human)

Additional COBRA1 Products

Product Documents for COBRA1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for COBRA1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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