COG3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-36540
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.1 mg/ml
Product Summary for COG3 Antibody
Immunogen
The immunogen recognized by the antibody maps to a region between residue 1 to 50 of human Component of Oligomeric Golgi Complex 3 using the numbering given in entry EAX08741.1.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for COG3 Antibody
![Western Blot: COG3 Antibody [NBP2-36540]](https://resources.bio-techne.com/images/products/COG3-Antibody-Western-Blot-NBP2-36540-img0001.jpg "Western Blot: COG3 Antibody [NBP2-36540]")
Western Blot: COG3 Antibody [NBP2-36540]
Western Blot: COG3 Antibody [NBP2-36540] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-COG3 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.![Immunoprecipitation: COG3 Antibody [NBP2-36540]](https://resources.bio-techne.com/images/products/COG3-Antibody-Immunoprecipitation-NBP2-36540-img0002.jpg "Immunoprecipitation: COG3 Antibody [NBP2-36540]")
Immunoprecipitation: COG3 Antibody [NBP2-36540]
Immunoprecipitation: COG3 Antibody [NBP2-36540] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. For blotting immunoprecipitated COG3, it was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for COG3 Antibody
Application
Recommended Usage
Immunoprecipitation
2 - 10 ug/mg
Western Blot
1:2000 - 1:10000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Validation by IP/Western Blot was performed using a 4-8% SDS-PAGE gel.
Validation by IP/Western Blot was performed using a 4-8% SDS-PAGE gel.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
0.1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: COG3
Alternate Names
COG complex subunit 3, component of oligomeric golgi complex 3p94, SEC34conserved oligomeric Golgi complex subunit 3, tethering factor SEC34, Vesicle-docking protein SEC34 homolog
Gene Symbol
COG3
UniProt
Additional COG3 Products
Product Documents for COG3 Antibody
Product Specific Notices for COG3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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