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COG6 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84411

Novus Biologicals, part of Bio-Techne
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NBP1-84411

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VQQHKPEQGSLANMPNLDSVTLKAAMVQFDRYLSAPDNLLIPQLNFLLSATVKEQIVKQSTELVCRAYGEVYAAVMNPINEYKDPENILHRSPQQVQTLL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (84%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for COG6 Antibody

Western Blot: COG6 Antibody [NBP1-84411]

Western Blot: COG6 Antibody [NBP1-84411]

Western Blot: COG6 Antibody [NBP1-84411] - Analysis in control (vector only transfected HEK293T lysate) and COG6 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: COG6 Antibody [NBP1-84411]

Immunocytochemistry/ Immunofluorescence: COG6 Antibody [NBP1-84411]

Immunocytochemistry/Immunofluorescence: COG6 Antibody [NBP1-84411] - Staining of human cell line A-431 shows positivity in nucleus but not nucleoli and golgi apparatus.
Immunohistochemistry-Paraffin: COG6 Antibody [NBP1-84411]

Immunohistochemistry-Paraffin: COG6 Antibody [NBP1-84411]

Immunohistochemistry-Paraffin: COG6 Antibody [NBP1-84411] - Staining of human testis shows strong cytoplasmic positivity in cells of seminiferous ducts.

Applications for COG6 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: COG6

Multiprotein complexes are key determinants of Golgi apparatus structure and its capacity for intracellular transport and glycoprotein modification. Several complexes have been identified, including the Golgi transport complex (GTC), the LDLC complex, which is involved in glycosylation reactions, and the SEC34 complex, which is involved in vesicular transport. These 3 complexes are identical and have been termed the conserved oligomeric Golgi (COG) complex, which includes COG6 (Ungar et al., 2002 [PubMed 11980916]).[supplied by OMIM]

Alternate Names

COG complex subunit 6, component of oligomeric golgi complex 6KIAA1134COD2complexed with Dor1p 2, conserved oligomeric Golgi complex protein 6, conserved oligomeric Golgi complex subunit 6, DKFZp313D191

Gene Symbol

COG6

Additional COG6 Products

Product Documents for COG6 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for COG6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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