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COG7 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47502

Novus Biologicals, part of Bio-Techne
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NBP2-47502

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: ISAKLTGMQNSLMMLVDTPDYSEKCVHLEALKNRLEALASPQIVAAFTSQAVDQSKVFVKVFTEIDRMPQLLAYYYKCHKVQLLAAWQELCQSDLSLDRQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for COG7 Antibody

Western Blot: COG7 Antibody [NBP2-47502]

Western Blot: COG7 Antibody [NBP2-47502]

Western Blot: COG7 Antibody [NBP2-47502] - Analysis in control (vector only transfected HEK293T lysate) and COG7 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: COG7 Antibody [NBP2-47502]

Immunocytochemistry/ Immunofluorescence: COG7 Antibody [NBP2-47502]

Immunocytochemistry/Immunofluorescence: COG7 Antibody [NBP2-47502] - Staining of human cell line A-431 shows localization to the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: COG7 Antibody [NBP2-47502]

Immunohistochemistry-Paraffin: COG7 Antibody [NBP2-47502]

Immunohistochemistry-Paraffin: COG7 Antibody [NBP2-47502] - Staining of human lateral ventricle shows strong cytoplasmic positivity in astrocytes.

Applications for COG7 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: COG7

Multiprotein complexes are key determinants of Golgi apparatus structure and its capacity for intracellular transport and glycoprotein modification. Several complexes have been identified, including the Golgi transport complex (GTC), the LDLC complex, which is involved in glycosylation reactions, and the SEC34 complex, which is involved in vesicular transport. These 3 complexes are identical and have been termed the conserved oligomeric Golgi (COG) complex, which includes COG7 (Ungar et al., 2002 [PubMed 11980916]).[supplied by OMIM]

Alternate Names

CDG2E, component of oligomeric golgi complex 7COG complex subunit 7, conserved oligomeric Golgi complex subunit 7

Gene Symbol

COG7

Additional COG7 Products

Product Documents for COG7 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for COG7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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