Coiled-Coil Domain Containing 149 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-30476
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (94%), Rat (97%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: RHQSLKKKYRELIDGDPSLPPEKRKQANLAQLLRDSQDRNKHLGEEIKELQQRLGEVQGDNKLLRMTIAKQRLGDEAIGVRHFAAHER
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Coiled-Coil Domain Containing 149 Antibody
Western Blot: Coiled-Coil Domain Containing 149 Antibody [NBP2-30476]
Western Blot: Coiled-Coil Domain Containing 149 Antibody [NBP2-30476] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissueImmunocytochemistry/ Immunofluorescence: Coiled-Coil Domain Containing 149 Antibody [NBP2-30476]
Immunocytochemistry/Immunofluorescence: Coiled-Coil Domain Containing 149 Antibody [NBP2-30476] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli.Immunohistochemistry: Coiled-Coil Domain Containing 149 Antibody [NBP2-30476]
Immunohistochemistry: Coiled-Coil Domain Containing 149 Antibody [NBP2-30476] - Staining of human kidney shows strong cytoplasmic and extracellular positivity in renal tubules.Applications for Coiled-Coil Domain Containing 149 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Coiled-Coil Domain Containing 149
Alternate Names
CCDC149, Coiled-Coil Domain-Containing Protein 149
Gene Symbol
CCDC149
UniProt
Additional Coiled-Coil Domain Containing 149 Products
Product Documents for Coiled-Coil Domain Containing 149 Antibody
Product Specific Notices for Coiled-Coil Domain Containing 149 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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