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Collagen II Antibody (HL1772) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25418

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25418

Key Product Details

Species Reactivity

Validated:

Human, Canine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1772

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for Collagen II Antibody (HL1772) - Azide and BSA Free

Immunogen

Recombinant fragment of human Collagen II

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Collagen II Antibody (HL1772) - Azide and BSA Free

Collagen II Antibody (HL1772) - Azide and BSA Free

Western Blot: Collagen II Antibody (HL1772) - Azide and BSA Free [NBP3-25418] -

Western Blot: Collagen II Antibody (HL1772) - Azide and BSA Free [NBP3-25418] - HepG2 whole cell extract and conditioned medium (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen II antibody [HL1772] (NBP3-25418) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Collagen II Antibody (HL1772) - Azide and BSA Free

Immunohistochemistry-Paraffin: Collagen II Antibody (HL1772) - Azide and BSA Free [NBP3-25418] -

Immunohistochemistry-Paraffin: Collagen II Antibody (HL1772) - Azide and BSA Free [NBP3-25418] - Collagen II antibody [HL1772] detects secreted Collagen II protein by immunohistochemical analysis. Sample: Paraffin-embedded dog cerebellum. Collagen II stained by Collagen II antibody [HL1772] (NBP3-25418) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Collagen II Antibody (HL1772) - Azide and BSA Free

Western Blot: Collagen II Antibody (HL1772) - Azide and BSA Free [NBP3-25418] -

Western Blot: Collagen II Antibody (HL1772) - Azide and BSA Free [NBP3-25418] - Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen II antibody [HL1772] (NBP3-25418) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Collagen II Antibody (HL1772) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:1000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Collagen II

The extensive family of COL gene products (collagens) is composed of several chain types, including fibril-forming interstitial collagens (types I, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue, such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Several collagens also play a role in cell adhesion, important for maintaining normal tissue architecture and function. In cartilage, Collagen Type II constitutes the bulk of the fibril. Sensitization with Collagen Type II induces an erosive polyarthritis in rats, mice and higher primates which can resemble rheumatoid arthritis and relapsing polychrondritis.

Alternate Names

Chondrocalcin, COL2A1, SEDC

Gene Symbol

COL2A1

Additional Collagen II Products

Product Documents for Collagen II Antibody (HL1772) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Collagen II Antibody (HL1772) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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