Collagen IV alpha5 Antibody (COL4A5/9396R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23832
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # COL4A5/9396R
Concentration
0.2 mg/ml
Product Summary for Collagen IV alpha5 Antibody (COL4A5/9396R)
Immunogen
Recombinant fragment (around aa1400-1685) of human Collagen IV alpha5 protein (exact sequence is proprietary)
Localization
Secreted. Basement membrane.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Description
Store at 2 to 8C. Non-hazardous. No MSDS required.
Scientific Data Images for Collagen IV alpha5 Antibody (COL4A5/9396R)
Immunohistochemistry-Paraffin: Collagen IV alpha5 Antibody (COL4A5/9396R) [NBP3-23832] -
Formalin-fixed, paraffin-embedded human pancreas stained with Collagen IV alpha5 antibody (COL4A5/9396R). Inset: PBS instead of primary antibody; secondary only negative control.Applications for Collagen IV alpha5 Antibody (COL4A5/9396R)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Collagen IV alpha5
Alternate Names
alpha-5 polypeptide, Alport syndrome, CA54, collagen of basement membrane, alpha-5 chain, collagen, type IV, alpha 5, dA149D17.3, dA24A23.1, MGC167109, MGC42377
Gene Symbol
COL4A5
Additional Collagen IV alpha5 Products
Product Documents for Collagen IV alpha5 Antibody (COL4A5/9396R)
Product Specific Notices for Collagen IV alpha5 Antibody (COL4A5/9396R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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