Collagen IV alpha5 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92585
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1462-1691 of human COL4A5 (NP_203699.1). SSVAHGFLITRHSQTTDAPQCPQGTLQVYEGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMSMQPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPHCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDVSDMFSKPQSETLKAGDLRTRISRCQVCMKRT
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
161 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Collagen IV alpha5 Antibody - BSA Free
![Western Blot: Collagen IV alpha5 AntibodyBSA Free [NBP2-92585]](https://resources.bio-techne.com/images/products/Collagen-IV-alpha5-Antibody-Western-Blot-NBP2-92585-img0001.jpg "Western Blot: Collagen IV alpha5 AntibodyBSA Free [NBP2-92585]")
Western Blot: Collagen IV alpha5 AntibodyBSA Free [NBP2-92585]
Western Blot: Collagen IV alpha5 Antibody [NBP2-92585] - Analysis of extracts of various cell lines, using Collagen IV alpha5 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.Applications for Collagen IV alpha5 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:500 - 1:1000
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS with 50% glycerol, pH7.3.
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Collagen IV alpha5
Alternate Names
alpha-5 polypeptide, Alport syndrome, CA54, collagen of basement membrane, alpha-5 chain, collagen, type IV, alpha 5, dA149D17.3, dA24A23.1, MGC167109, MGC42377
Gene Symbol
COL4A5
Additional Collagen IV alpha5 Products
Product Documents for Collagen IV alpha5 Antibody - BSA Free
Product Specific Notices for Collagen IV alpha5 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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