Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # rCOL4/4742

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Collagen IV Antibodies »

Product Specifications

Immunogen

Purified human placental extract

Localization

Secreted. Extracellular matrix.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

161 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07596).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Collagen IV Antibody (rCOL4/4742) - Azide and BSA Free

Immunohistochemistry-Paraffin: Collagen IV Antibody (rCOL4/4742) - Azide and BSA Free [NBP3-08487]

Immunohistochemistry-Paraffin: Collagen IV Antibody (rCOL4/4742) - Azide and BSA Free [NBP3-08487]

Immunohistochemistry-Paraffin: Collagen IV Antibody (rCOL4/4742) - Azide and BSA Free [NBP3-08487] - Formalin-fixed, paraffin-embedded human kidney stained with Collagen IV Recombinant Mouse Monoclonal Antibody (rCOL4/4742)
Immunohistochemistry-Paraffin: Collagen IV Antibody (rCOL4/4742) - Azide and BSA Free [NBP3-08487]

Immunohistochemistry-Paraffin: Collagen IV Antibody (rCOL4/4742) - Azide and BSA Free [NBP3-08487]

Immunohistochemistry-Paraffin: Collagen IV Antibody (rCOL4/4742) - Azide and BSA Free [NBP3-08487] - Formalin-fixed, paraffin-embedded human kidney stained with Collagen IV Recombinant Mouse Monoclonal Antibody (rCOL4/4742).

Applications for Collagen IV Antibody (rCOL4/4742) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Collagen IV

Collagens comprise a large family of insoluble extracellular glycoproteins that are essential components of connective tissues such as tendons, ligaments, cartilage, bone and skin. The mature polypeptides are secreted as coiled, left-handed helices that subsequently assemble into rope-like collagen fibers. Collagen I is a fibril-forming collagen that requires N- and C- terminal processing. Collagen IV is a network forming collagen whose C-terminus forms dimers and N-terminus forms tetramers.

Alternate Names

BSVD;BSVD1;COL4A1s;Collagen alpha-1(IV) chain;PADMAL;RATOR

Gene Symbol

COL4A1

Additional Collagen IV Products

Product Documents for Collagen IV Antibody (rCOL4/4742) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Collagen IV Antibody (rCOL4/4742) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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