Collagen IX alpha 2 Antibody

Immunohistochemistry: Collagen IX alpha 2 Antibody [NBP2-30450]

Immunohistochemistry: Collagen IX alpha 2 Antibody [NBP2-30450] - Immunofluorescence assay of COL9 alpha2 (h) of the distal femurs of 3-day-old WT and Prx1Cre, Piezo1fl/fl mice. Scale bar = 100 um. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-019-14146-6) licensed under a CC-BY license.

Immunocytochemistry/ Immunofluorescence: Collagen IX alpha 2 Antibody [NBP2-30450]

Immunocytochemistry/Immunofluorescence: Collagen IX alpha 2 Antibody [NBP2-30450] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & vesicles. Antibody staining is shown in green.

Immunocytochemistry/ Immunofluorescence: Collagen IX alpha 2 Antibody [NBP2-30450] -

Immunocytochemistry/ Immunofluorescence: Collagen IX alpha 2 Antibody [NBP2-30450] - Col2 alpha1 & Col9 alpha2 mediated the inhibition of bone resorption by PIEZO1. (g, h) Immunofluorescence assay of COL2 alpha1 (g) & COL9 alpha2 (h) of distal femurs of 3-day-old WT & Prx1Cre, Piezo1fl/fl mice. Scale bar = 100 μm. i, j QPCR analysis of Col2 alpha1 (i) & Col9 alpha2 (j) in BMSCs-derived osteoblasts from WT & Prx1Cre, Piezo1fl/fl mice endured w/ 0.5 Hz, 1% intensity compression for 4 h by FlexCell compression system. *P < 0.05; **P < 0.01. Ordinary one-way ANOVA. Data are mean ± SD, n = 4. k–l Osteoclastogenesis by OB-OC co-culture in vitro using BMSCs-derived osteoblasts from WT & Prx1Cre, Piezo1fl/fl mice infected w/ Ctrl, Col2 alpha1 & Col9 alpha2 lenti-virus, respectively. k TRAP staining of osteoclasts in co-culture system. Scale bar = 100 μm. l Co-culture supernatants measured for TRAP activity via colorimetric readout (A405). *P < 0.05; **P < 0.01. Ordinary one-way ANOVA. Data are mean ± SD, n = 3. Source data are provided in Source Data File. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31941964), licensed under a CC-BY license. Not internally tested by Novus Biologicals.