Collagen X alpha 1 Antibody (0F5Q9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15419
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 0F5Q9
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Collagen X alpha 1 Antibody (0F5Q9)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 500-600 of human Collagen X alpha 1 (Q03692). HSGEPGLPGPPGPPGPPGQAVMPEGFIKAGQRPSLSGTPLVSANQGVTGMPVSAFTVILSKAYPAIGTPIPFDKILYNRQQHYDPRTGIFTCQIPGIYYFS
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Collagen X alpha 1 Antibody (0F5Q9)
![Western Blot: Collagen X alpha 1 Antibody (0F5Q9) [NBP3-15419]](https://resources.bio-techne.com/images/products/Collagen-X-alpha-1-Antibody-0F5Q9-Western-Blot-NBP3-15419-img0001.jpg "Western Blot: Collagen X alpha 1 Antibody (0F5Q9) [NBP3-15419]")
Western Blot: Collagen X alpha 1 Antibody (0F5Q9) [NBP3-15419]
Western Blot: Collagen X alpha 1 Antibody (0F5Q9) [NBP3-15419] - Western blot analysis of extracts of various cell lines, using Collagen X alpha 1 Rabbit mAb (NBP3-15419) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.Applications for Collagen X alpha 1 Antibody (0F5Q9)
Application
Recommended Usage
Western Blot
1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Collagen X alpha 1
Alternate Names
COL10A1, Collagen Alpha-1(X) Chain, Collagen X, Alpha-1 Polypeptide, Schmid Metaphyseal Chondrodysplasia
Gene Symbol
COL10A1
Additional Collagen X alpha 1 Products
Product Documents for Collagen X alpha 1 Antibody (0F5Q9)
Product Specific Notices for Collagen X alpha 1 Antibody (0F5Q9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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