Collagen X alpha 1 Antibody (JF0961)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-66988
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JF0961
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within Human Collagen X alpha 1 aa 550-599 / 680. (SwissProt: Q03692 Human; SwissProt: Q05306 Mouse; Unigene:20579 Rat)
Localization
Extracellular matrix.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Collagen X alpha 1 Antibody (JF0961)
![Western Blot: Collagen X alpha 1 Antibody (JF0961) [NBP2-66988]](https://resources.bio-techne.com/images/products/Collagen-X-alpha-1-Antibody-JF0961-Western-Blot-NBP2-66988-img0002.jpg "Western Blot: Collagen X alpha 1 Antibody (JF0961) [NBP2-66988]")
Western Blot: Collagen X alpha 1 Antibody (JF0961) [NBP2-66988]
Western Blot: Collagen X alpha 1 Antibody (JF0961) [NBP2-66988] - Analysis of Collagen X alpha 1 on rat liver tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.![Western Blot: Collagen X alpha 1 Antibody (JF0961) [NBP2-66988]](https://resources.bio-techne.com/images/products/Collagen-X-alpha-1-Antibody-JF0961-Western-Blot-NBP2-66988-img0001.jpg "Western Blot: Collagen X alpha 1 Antibody (JF0961) [NBP2-66988]")
Western Blot: Collagen X alpha 1 Antibody (JF0961) [NBP2-66988]
Western Blot: Collagen X alpha 1 Antibody (JF0961) [NBP2-66988] - Analysis of Collagen X on human skin lysates using anti-Collagen X antibody at 1/1,000 dilution.![Western Blot: Collagen X alpha 1 Antibody (JF0961) [NBP2-66988]](https://resources.bio-techne.com/images/products/Collagen-X-alpha-1-Antibody-JF0961-Western-Blot-NBP2-66988-img0003.jpg "Western Blot: Collagen X alpha 1 Antibody (JF0961) [NBP2-66988]")
Western Blot: Collagen X alpha 1 Antibody (JF0961) [NBP2-66988]
Western Blot: Collagen X alpha 1 Antibody (JF0961) [NBP2-66988] - Analysis of Collagen X alpha 1 on human liver tissue lysates with Rabbit anti-Collagen X alpha 1 antibody at 1/1,000 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.Applications for Collagen X alpha 1 Antibody (JF0961)
Application
Recommended Usage
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Collagen X alpha 1
Alternate Names
COL10A1, Collagen Alpha-1(X) Chain, Collagen X, Alpha-1 Polypeptide, Schmid Metaphyseal Chondrodysplasia
Gene Symbol
COL10A1
Additional Collagen X alpha 1 Products
Product Documents for Collagen X alpha 1 Antibody (JF0961)
Product Specific Notices for Collagen X alpha 1 Antibody (JF0961)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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