Collagen XXV alpha 1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB300-249

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Collagen XXV alpha 1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide mapping to residues 171-183 [NHGFLSADQQLIK] of the NC2-1 region of human and mouse CLAC-P.

Reactivity Notes

Human and mouse. Other species have not been tested.

Specificity

Reacts with residues 171-183 [NHGFLSADQQLIK] of the NC2-1 region of human and mouse CLAC-P. Detects bands of 50, 70 and 100 kDa in SPs of AD brain, but not in diffuse plaques of non-demented aged individuals, diffuse deposits in sub-cortical structures and cerebellum in AD nor in cerebral amyloid angiopathy.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Collagen XXV alpha 1 Antibody - BSA Free

Western Blot: Collagen XXV alpha 1 AntibodyBSA Free [NB300-249]

Western Blot: Collagen XXV alpha 1 AntibodyBSA Free [NB300-249]

Western Blot: Collagen XXV Antibody [NB300-249] - Analysis of Collagen XXV, using Img-4153 and IMG-4154 at 1:500 dilution on 100 ug of formic acid extracts of Alzheimer diseased brain.

Applications for Collagen XXV alpha 1 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:100-1:2000
Application Notes
Western blot - Use at 1:500 dilution. This antibody has only been tested in Western Blot. The investigator should determine the optimal dilution for a specific application such as immunohistochemistry.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Collagen XXV alpha 1

Antibodies against senile plaque (SP) amyloid obtained a clone 9D2, which labeled amyloid fibrils in SPs and reacted with approximately 50/100 kDa polypeptides in Alzheimer's disease (AD) brains. The 9D2 antigen was identified as CLAC (collagen-like Alzheimer amyloid plaque component), and its precursor as CLAC-P/collagen type XXV. Both secreted and membrane-tethered forms of CLAC-P/collagen type XXV specifically bound to fibrillized Abeta, implicating these proteins in beta-amyloidogenesis and neuronal degeneration in AD.

Alternate Names

COL25A1

Entrez Gene IDs

84570 (Human); 77018 (Mouse)

Gene Symbol

COL25A1

Additional Collagen XXV alpha 1 Products

Product Documents for Collagen XXV alpha 1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Collagen XXV alpha 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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