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COMMD8 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82178

Novus Biologicals, part of Bio-Techne
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NBP1-82178
NBP1-82178-25ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for COMMD8 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SRKDEIKQALSREIVAISSAQLQDFDWQVKLALSSDKIAALRMPLLSLHLDVKENGEVKPYSIEMSREELQNLIQSLEAANKVVLQLK

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%), Rat (84%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for COMMD8 Antibody

Western Blot: COMMD8 Antibody [NBP1-82178]

Western Blot: COMMD8 Antibody [NBP1-82178]

Western Blot: COMMD8 Antibody [NBP1-82178] - Analysis in control (vector only transfected HEK293T lysate) and COMMD8 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: COMMD8 Antibody [NBP1-82178]

Immunocytochemistry/ Immunofluorescence: COMMD8 Antibody [NBP1-82178]

Immunocytochemistry/Immunofluorescence: COMMD8 Antibody [NBP1-82178] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: COMMD8 Antibody [NBP1-82178]

Immunohistochemistry-Paraffin: COMMD8 Antibody [NBP1-82178]

Immunohistochemistry-Paraffin: COMMD8 Antibody [NBP1-82178] - Staining of human thyroid gland shows moderate cytoplasmic positivity in glandular cells.

Applications for COMMD8 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: COMMD8

Alternate Names

COMM domain containing 8, COMM domain-containing protein 8, FLJ20502

Gene Symbol

COMMD8

Additional COMMD8 Products

Product Documents for COMMD8 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for COMMD8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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