Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA Capture, Western Blot

Label

Janelia Fluor 635

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # K13/16-5.7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Complement C3a Antibodies »

Product Specifications

Immunogen

Complement C3a Antibody (K13/16-5.7) - BSA Free was developed against Human C3a.

Epitope

No reaction is seen with a synthetic octapeptide representing the C3a C-terminal.

Specificity

Complement C3a Antibody (K13/16-5.7) - BSA Free is specific for human C3, C3a and C3a (desArg) and does not cross-react with C4a or C5a.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Applications for Complement C3a Antibody (K13/16-5.7) [Janelia Fluor® 635]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA Capture

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Complement C3a

The complement system, or complement cascade, is a part of the innate immune system that assists in defense against pathogens (1). Complement C3, also called C3 or C3 protein, is one of nine complement proteins and is the main component of the complement system which is composed of over 30 soluble and membrane-bound proteins (1). C3 is cleaved by C3 convertase into two fragments, the smaller C3a and larger C3b (1,2). Human C3a is 77 amino acids (aa) in length and has a theoretical molecular weight of ~9 kDa (2). Structurally, complement C3a consists of four anti-parallel helical structures with three disulfide bridges (2). The C3a fragment binds the G-protein coupled receptor (GPCR) C3a (C3aR) (2,3). Functionally, C3a plays an important role in regulating both adaptive immunity and innate immunity (1-3).

The C3a/C3aR pathway is often referred to as a "double-edged sword" because it has both anti-inflammatory and pro-inflammatory functions (2,3). For instance, its anti-inflammatory role includes pathogen elimination and reduced interferon-gamma (IFN-gamma) expression and cytotoxicity in natural killer cells (2). By contrast, complement C3a is an anaphylatoxin and plays a role in inflammation by prompting histamine release from mast cells and increasing permeability of the vascular system (2-4). The C3a/C3aR pathway has also been indicated in a number of diseases including Alzheimer's disease, asthma, and kidney disease (3). Patients with various kidney diseases tend to show higher levels of complement C3a in the plasma and urine (3).

References

1. Mathern, D. R., & Heeger, P. S. (2015). Molecules Great and Small: The Complement System. Clinical Journal of the American Society of Nephrology: CJASN. https://doi.org/10.2215/CJN.06230614

2. Gao, S., Cui, Z., & Zhao, M. H. (2020). The Complement C3a and C3a Receptor Pathway in Kidney Diseases. Frontiers in Immunology. https://doi.org/10.3389/fimmu.2020.01875

3. Ricklin, D., Reis, E. S., Mastellos, D. C., Gros, P., & Lambris, J. D. (2016). Complement component C3 - The "Swiss Army Knife" of innate immunity and host defense. Immunological Reviews. https://doi.org/10.1111/imr.12500

4. Haddad, A., & Wilson, A. M. (2020). Biochemistry, Complement. In StatPearls. StatPearls Publishing.

Alternate Names

Acylation Stimulating Protein, acylation-stimulating protein cleavage product, AHUS5, ARMD9, ASP, C3, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, C3a, C3a anaphylatoxin, C3adesArg, C3b, C3bc, C3-beta-c, complement C3, Complement C3 alpha chain, Complement C3 beta chain, Complement C3b alpha' chain, Complement C3c alpha' chain fragment 1, Complement C3c alpha' chain fragment 2, Complement C3d fragment, Complement C3dg fragment, Complement C3f fragment, Complement C3g fragment, complement component 3, complement component C3, complement component C3a, complement component C3b, CPAMD1, EC 3.4.21.43, epididymis secretory sperm binding protein Li 62p, HEL-S-62p, prepro-C3

Gene Symbol

C3

Additional Complement C3a Products

Product Documents for Complement C3a Antibody (K13/16-5.7) [Janelia Fluor® 635]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Complement C3a Antibody (K13/16-5.7) [Janelia Fluor® 635]



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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