Complement C4d Antibody (12D11)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62802

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Porcine, Bovine, Monkey, Primate

Applications

Flow Cytometry, Immunoassay, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 12D11

Concentration

0.1 mg/ml

View all Complement C4d Antibodies »

Product Specifications

Immunogen

Compliment factor C4D

Epitope

The monoclonal antibody 12D11 (also known as clone M4d3) recognizes an epitope on the alpha 2 chain of human C4d.

Specificity

This antibody recognizes C4, C4b and C4d and is predicted to cross react with pig, bovine, baboon and rhesus monkey.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Complement C4d Antibody (12D11)

Immunohistochemistry-Frozen: Complement C4d Antibody (12D11) [NBP2-62802]

Immunohistochemistry-Frozen: Complement C4d Antibody (12D11) [NBP2-62802]

Immunohistochemistry-Frozen: Complement C4d Antibody (12D11) [NBP2-62802] - IHC-F of human tonsil using this antibody.

Applications for Complement C4d Antibody (12D11)

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry-Frozen

1:10-1:500

Western Blot

1:100-1:2000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS with 0.1% BSA

Preservative

0.02% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Complement C4d

Complement factor C4, formerly known as Gg protein, consists of an alpha-, beta-, and gamma-chain. The classical pathway of complement and the mannose binding lectin (MBL) activation pathway converge at C4. C1s, MASP-1 and MASP-2 cleave C4 resulting in the formation of C4a and C4b. The latter can be cleaved resulting in C4c and C4d, in which step all functional sites are lost. Therefore, the C4d activation fragment of C4 is an excellent marker for classical complement pathway and MBL pathway activation. In a number of diseases such as rheumatoid arthritis (RA), hereditary angioedema (HAE), systemic lupus erythematosus (SLE) and chronic urticaria with hypercomplementemia levels of C4d are significantly elevated in serum or plasma. C4d levels may also be elevated in plasma from patients with a variety of humoral autoimmune diseases in which complement activation is known to occur. Deposition of C4d in peritubular capillaries has been shown to be a sensitive marker for antibody-mediated (humoral) rejection in renal transplant biopsies.

Alternate Names

C4, C4A, C4A2, C4A3, C4A4, C4A6, C4S, CO4, complement C4A, CPAMD2, RG

Gene Symbol

C4A

Additional Complement C4d Products

Product Documents for Complement C4d Antibody (12D11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Complement C4d Antibody (12D11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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