Complement C8A/B/G Antibody (056B-373)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-64340

Novus Biologicals, part of Bio-Techne
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NB100-64340
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 056B-373

Concentration

1.0 mg/ml

View all Complement C8A/B/G Antibodies »

Product Specifications

Immunogen

Purified human C8

Specificity

This antibody recognizes complement component 8 (C8), a 151 kDa member of the complement C6/C7/C8/C9 family, present in blood serum. C8 is a terminal component of the complement system, part of both the complement membrane attack complex (MAC), and important to MAC assembly. C8 binds to the C5b-7 complex, anchored to the membrane, creating C5b-8. C5b-8 binds C9 and catalyzes the polymerization of C9 molecules to form C5-b9 (MAC). C8 is thought to contain lipid binding sites, facilitating the insertion of MAC into the membrane. Defects in the alpha chain of C8 can result in complement C8 deficiency type I. Furthermore, C8 deficiencies can cause recurring bacterial infections, in particular from Neisseria meningitides.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Complement C8A/B/G Antibody (056B-373)

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Western Blot

1:100-1:2000

Reviewed Applications

Read 1 review rated 5 using NB100-64340 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Borate buffered saline (pH 8.2 - 8.4)

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Complement C8A/B/G

C8 is synthesised in the liver, monocytes and fibroblasts and is a plasma protein consisting of three non identical polypeptide chains. The alpha-chain (64kDa) and gamma chain (22kDa) are disulphide linked, these associate non-covalently with the beta-chain (64kDa), to form the native C8 molecule with a molecular weight of 151kDa. Normal plasma concentration is 80mg/L. C8 is one of the terminal proteins of the complement pathways. C8 binds to the membrane bound C5b-7, C5b-8 combines with C9 to form the membrane attack complex (MAC). The MAC forms a hydrophilic, protein walled, transmembrane channel, formation of which leads to cell lysis and destruction.

Alternate Names

complement component 8, alpha polypeptide, complement component C8 alpha chain

Entrez Gene IDs

731 (Human)

Gene Symbol

C8A

UniProt

P07360 (Human)

Additional Complement C8A/B/G Products

Product Documents for Complement C8A/B/G Antibody (056B-373)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Complement C8A/B/G Antibody (056B-373)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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