Complement Component C1qA Antibody (JL-1) [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51140B

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Flow Cytometry, Functional, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry

Label

Biotin

Antibody Source

Monoclonal Mouse IgG2B Clone # JL-1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Complement Component C1qA Antibodies »

Product Summary for Complement Component C1qA Antibody (JL-1) [Biotin]

Immunogen

Purified mouse C1QA

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

Positive control: Spleen and kidney tissue of wild-type mice (Ref.1) Negative control: Spleen and kidney tissue of C1q -/- mice (Ref.1)

Applications for Complement Component C1qA Antibody (JL-1) [Biotin]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um filtered biotinylated antibody solution in PBS and 0.1% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Complement Component C1qA

The monoclonal antibody JL-1 recognizes the collagen-like region (CLR) of mouse C1q, a 459 kDa molecule consisting of three individual polypeptide chains. The antibody has been generated by immunization of C1q-/- C57BL/6 mice with purified mouse C1q. C1q forms together with C1r and C1s the C1 macromolecule, the first component of the classical complement pathway. Interaction of immune complexes with C1q induces a conformational change within the C1 complex, which results in activation of the classical pathway. C1q functions as recognition unit by binding to the heavy chain of IgG or IgM (Fc gamma and Fc micro) provided that the immunoglobulins are bound to their antigen. Furthermore, C1q can also recognize molecular patterns associated with pathogens and it can bind to apoptotic blebs, where it activates the classical complement pathway and mediates phagocytosis. As such, C1q promotes the clearance of apoptotic cells and subsequent exposure of auto antigens, thereby preventing stimulation of the immune system. C1q is predominantly produced by macrophages but also by follicular dendritic cells, interdigitating cells and cells of the monocyte-macrophage lineage. C1q deficiency has a profound effect on host defence and clearance of immune complexes. Absence of C1q may cause autoimmunity by impairment of the clearance of apoptotic cells. Inherited C1q deficiency is also associated with the development of systemic lupus erythematosus (SLE). The monoclonal antibody JL-1 is reactive with the collagen-like region (CLR) only, which is the same region to which autoantibodies in mice and humans are binding. Anti-C1q autoantibodies deposit in glomeruli together with C1q but induce overt renal disease only in the context of glomerular immune complex disease. This provides an explanation why anti-C1q antibodies are especially pathogenic in patients with SLE.

Long Name

Complement Component 1q Subcomponent, A Chain

Alternate Names

C1QA

Gene Symbol

C1QA

Additional Complement Component C1qA Products

Product Documents for Complement Component C1qA Antibody (JL-1) [Biotin]

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Complement Component C1qA Antibody (JL-1) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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