Complement Component C5/C5a Antibody (06)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06182

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Sandwich ELISA Capture

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # 06

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Complement Component C5/C5a Antibodies »

Product Summary for Complement Component C5/C5a Antibody (06)

Immunogen

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Complement Component C5/C5a (Accession#: NP_001726.2; Leu679-Arg751).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Applications for Complement Component C5/C5a Antibody (06)

Application
Recommended Usage

Sandwich ELISA Capture

1:250-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Complement Component C5/C5a

Human complement component C5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as anaphylatoxins. C5a is a 74 amino acid (aa) peptide that is created by the activity of C5a convertase on the C5 alpha-chain. Human C5a has four alpha-helices plus three intrachain disulfide bonds that create a triple loop structure. In serum, proteolytic processing removes the C-terminal arginine, creating a low activity C5a desArg74 molecule. Human C5a is 60% and 54% aa identical to mouse and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (C5aR/CD88) and a non-signaling GPCR termed C5L2. Activation of CD88 results in neutrophil chemotaxis and endothelial cell activation. It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells.

Alternate Names

C5

Gene Symbol

C5

Additional Complement Component C5/C5a Products

Product Documents for Complement Component C5/C5a Antibody (06)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Complement Component C5/C5a Antibody (06)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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