Complement Factor I Antibody (1B3)

Novus Biologicals, part of Bio-Techne | Catalog # H00003426-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 1B3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Complement Factor I Antibodies »

Product Specifications

Immunogen

IF (NP_000195, 19 a.a. ~ 118 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KVTYTSQEDLVEKKCLAKKYTHLSCDKVFCQPWQRCIEGTCVCKLPYQCPKNGTAVCATNRRSFPTYCQQKSLECLHPGTKFLNNGTCTAEGKFSVSLKH

Specificity

IF (1B3)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Complement Factor I Antibody (1B3)

Western Blot: Complement Factor I Antibody (1B3) [H00003426-M01]

Western Blot: Complement Factor I Antibody (1B3) [H00003426-M01]

Western Blot: Complement Factor I Antibody (1B3) [H00003426-M01] - Analysis of CFI expression in transfected 293T cell line by IF monoclonal antibody (M01), clone 1B3.Lane 1: CFI transfected lysate (Predicted MW: 42.4 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: Complement Factor I Antibody (1B3) [H00003426-M01] - Detection limit for recombinant GST tagged IF is approximately 0.3ng/ml as a capture antibody.

Applications for Complement Factor I Antibody (1B3)

Application
Recommended Usage

Sandwich ELISA

1:100-1:2000

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Complement Factor I

This gene encodes a serine proteinase that is essential for regulating the complement cascade. The encoded preproprotein is cleaved to produce both heavy and light chains, which are linked by disulfide bonds to form a heterodimeric glycoprotein. This heterodimer can cleave and inactivate the complement components C4b and C3b, and it prevents the assembly of the C3 and C5 convertase enzymes. Defects in this gene cause complement factor I deficiency, an autosomal recessive disease associated with a susceptibility to pyogenic infections. Mutations in this gene have been associated with a predisposition to atypical hemolytic uraemic syndrome, a disease characterized by acute renal failure, microangiopathic hemolytic anemia and thrombocytopenia. Primary glomerulonephritis with immmune deposits is another condition associated with mutation of this gene.

Alternate Names

C3b Inactivator, CFI

Entrez Gene IDs

3426 (Human)

Gene Symbol

CFI

OMIM

217030 (Human)

UniProt

NP_000195 (Human)

Additional Complement Factor I Products

Product Documents for Complement Factor I Antibody (1B3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Complement Factor I Antibody (1B3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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