Complexin-2 Antibody (021)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90019

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP2-90019-100ul
NBP2-90019-50ul
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 021

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Complexin-2 Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human Complexin-2 (Uniprot#: Q6PUV4-1; Asp2-Lys134).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Complexin-2 Antibody (021)

Immunocytochemistry/ Immunofluorescence: Complexin-2 Antibody (021) [NBP2-90019]

Immunocytochemistry/ Immunofluorescence: Complexin-2 Antibody (021) [NBP2-90019]

Immunocytochemistry/Immunofluorescence: Complexin-2 Antibody (021) [NBP2-90019] - Staining of CPLX2 in SHSY5Y cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human CPLX2 monoclonal antibody (1:60) at 4? overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green).
Flow Cytometry: Complexin-2 Antibody (021) [NBP2-90019]

Flow Cytometry: Complexin-2 Antibody (021) [NBP2-90019]

Flow Cytometry: Complexin-2 Antibody (021) [NBP2-90019] - Analysis of Human CPLX2 expression on SHSY5Y cells. The cells were treated, stained with purified anti-Human CPLX2, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Applications for Complexin-2 Antibody (021)

Application
Recommended Usage

Flow Cytometry

1:25-1:100

Immunocytochemistry/ Immunofluorescence

1:20-1:100

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Complexin-2

Proteins encoded by the complexin/synaphin gene family are cytosolic proteins that function in synaptic vesicle exocytosis. These proteins bind syntaxin, part of the SNAP receptor. The protein product of this gene binds to the SNAP receptor complex and disrupts it, allowing transmitter release. Two transcript variants encoding the same protein have been found for this gene. (provided by RefSeq)

Alternate Names

CPLX2, CPX-II, CPX2, Hfb1, Synaphin 1

Gene Symbol

CPLX2

Additional Complexin-2 Products

Product Documents for Complexin-2 Antibody (021)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Complexin-2 Antibody (021)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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