COMT Antibody (8E1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26646

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8E1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all COMT Antibodies »

Product Summary for COMT Antibody (8E1)

Immunogen

A synthesized peptide derived from Human COMT [UniProt P21964]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for COMT Antibody (8E1)

COMT Antibody (8E1)

Immunohistochemistry: COMT Antibody (8E1) [NBP3-26646] -

Immunohistochemistry: COMT Antibody (8E1) [NBP3-26646] - Image of COMT Antibody (8E1) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
COMT Antibody (8E1)

Western Blot: COMT Antibody (8E1) [NBP3-26646] -

Western Blot: COMT Antibody (8E1) [NBP3-26646] - Positive Western Blot detected in: 293 whole cell lysate, THP-1 whole cell lysate, HepG2 whole cell lysate.
All lanes: COMT Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 31, 25 kDa
Observed band size: 31 kDa

Applications for COMT Antibody (8E1)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: COMT

Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. The transcript variants are formed through the use of alternative translation initiation sites and promoters.

Long Name

Catechol O-Methyltransferase

Alternate Names

catechol O-methyltransferase, catechol-O-methyltransferase, EC 2.1.1.6

Gene Symbol

COMT

Additional COMT Products

Product Documents for COMT Antibody (8E1)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for COMT Antibody (8E1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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