COMT Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-41387

Novus Biologicals, part of Bio-Techne
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NB100-41387
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all COMT Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QDIIPQLKKKYDVD corresponding to internal region according to NP_000745.1, NP_009294.1.

Specificity

This antibody is expected to recognize both reported isoforms (NP_000745.1 and NP_009294.1)

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for COMT Antibody

Western Blot: COMT Antibody [NB100-41387]

Western Blot: COMT Antibody [NB100-41387]

Western Blot: COMT Antibody [NB100-41387] - Staining of Human Testis lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: COMT Antibody [NB100-41387]

Immunohistochemistry-Paraffin: COMT Antibody [NB100-41387]

Immunohistochemistry-Paraffin: COMT Antibody [NB100-41387] - Staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Antibody at 3.75 ug/mL.
Immunohistochemistry-Paraffin: COMT Antibody [NB100-41387]

Immunohistochemistry-Paraffin: COMT Antibody [NB100-41387]

Immunohistochemistry-Paraffin: COMT Antibody [NB100-41387] - Staining of paraffin embedded Human Cerebellum. Antibody at 4 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
View 1 more images

Applications for COMT Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

2 - 4 ug/mL

Peptide ELISA

Detection limit 1:64000

Western Blot

0.03 - 0.1 ug/mL
Application Notes
Western blot: Approx 28 kDa band in Human Testis lysates (calculated MW of 30.0 kDa according to NP_000745.1 and 24.5 kDa according to NP_009294.1). IHC: In paraffin embedded Human Brain (Cerebellum) shows staining of processes coming from the edge of the granular zone into the molecular layer. Recommended concentration: 2 - 4 ug/mL. Paraffin embedded Human Testis and Small Intestine. Recommended concentration: 3.75 ug/mL.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: COMT

Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. The transcript variants are formed through the use of alternative translation initiation sites and promoters.

Long Name

Catechol O-Methyltransferase

Alternate Names

catechol O-methyltransferase, catechol-O-methyltransferase, EC 2.1.1.6

Entrez Gene IDs

1312 (Human)

Gene Symbol

COMT

UniProt

NP_000745.1 (Human)

Additional COMT Products

Product Documents for COMT Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for COMT Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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