COMT Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85818

Novus Biologicals, part of Bio-Techne
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NBP1-85818
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all COMT Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: YCEQKEWAMNVGDKKGKIVDAVIQEHQPSVLLELGAYCGYSAVRMARLLSPGARLITIEINPDCAAITQRMVDFAGVKDKVTLVVGASQDIIPQLKKKYDVDTLDMVFLDHWKDRYLPDTLLLEECGLLRKGTVLLADNVICPGAPDFLAHVRGSSCFECTHYQSFLEYREVVDGLEKAIY

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for COMT Antibody

Western Blot: COMT Antibody [NBP1-85818]

Western Blot: COMT Antibody [NBP1-85818]

Western Blot: COMT Antibody [NBP1-85818] - Analysis in human cell lines MCF-7 and PC-3 using Anti-COMT antibody. Corresponding COMT RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.
Immunohistochemistry-Paraffin: COMT Antibody [NBP1-85818]

Immunohistochemistry-Paraffin: COMT Antibody [NBP1-85818]

Immunohistochemistry-Paraffin: COMT Antibody [NBP1-85818] - Staining in human placenta and pancreas tissues using anti-COMT antibody. Corresponding COMT RNA-seq data are presented for the same tissues.
Western Blot: COMT Antibody [NBP1-85818]

Western Blot: COMT Antibody [NBP1-85818]

Western Blot: COMT Antibody [NBP1-85818] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
View 2 more images

Applications for COMT Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: COMT

Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. The transcript variants are formed through the use of alternative translation initiation sites and promoters.

Long Name

Catechol O-Methyltransferase

Alternate Names

catechol O-methyltransferase, catechol-O-methyltransferase, EC 2.1.1.6

Gene Symbol

COMT

Additional COMT Products

Product Documents for COMT Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for COMT Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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