Connexin 26/GJB2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-57840

Novus Biologicals, part of Bio-Techne
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NB100-57840
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Canine (100%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Connexin 26/GJB2 Antibodies »

Product Specifications

Immunogen

Peptide with sequence YLLIRYCSGKSKKP corresponding to C-Terminus according to NP_003995.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Connexin 26/GJB2 Antibody

Western Blot: Connexin 26/GJB2 Antibody [NB100-57840]

Western Blot: Connexin 26/GJB2 Antibody [NB100-57840]

Western Blot: Connexin 26/GJB2 Antibody [NB100-57840] - Staining of Mouse Brain lysate with antibody at 0.01 ug/mL (A), Mouse Heart (B) and Rat Heart (C) lysate with antibody at 0.5 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Flow Cytometry: Connexin 26/GJB2 Antibody [NB100-57840]

Flow Cytometry: Connexin 26/GJB2 Antibody [NB100-57840]

Flow Cytometry: Connexin 26/GJB2 Antibody [NB100-57840] - Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Western Blot: Connexin 26/GJB2 Antibody [NB100-57840]

Western Blot: Connexin 26/GJB2 Antibody [NB100-57840]

Western Blot: Connexin 26/GJB2 Antibody [NB100-57840] - Staining of Human Colon (A), Heart (B), Prostate (C), Skin (D) and negative control Adipose (E) lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.

Applications for Connexin 26/GJB2 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Peptide ELISA

Detection limit 1:128000

Western Blot

0.01 - 1 ug/mL
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Connexin 26/GJB2

Gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell channels. Proteins, called connexins, purified from fractions of enriched gap junctions from different tissues differ. The connexins are designated by their molecular mass. Another system of nomenclature divides gap junction proteins into 2 categories, alpha and beta, according to sequence similarities at the nucleotide and amino acid levels. For example, CX43 (MIM 121014) is designated alpha-1 gap junction protein, whereas CX32 (GJB1; MIM 304040) and CX26 are called beta-1 and beta-2 gap junction proteins, respectively. This nomenclature emphasizes that CX32 and CX26 are more homologous to each other than either of them is to CX43.[supplied by OMIM]

Alternate Names

CX26, DFNA3, DFNB1, GJB2, HID, KID, NSRD1, PPK

Entrez Gene IDs

2706 (Human); 14619 (Mouse); 394266 (Rat)

Gene Symbol

GJB2

UniProt

P29033 (Human)

Additional Connexin 26/GJB2 Products

Product Documents for Connexin 26/GJB2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Connexin 26/GJB2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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